LuxS influences Escherichia coli biofilm formation through autoinducer-2-dependent and autoinducer-2-independent modalities

FEMS Microbiology Ecology
Chen NiuEric S Gilbert

Abstract

Escherichia coli produces biofilms in response to the small molecule autoinducer-2 (AI-2), a product of the LuxS enzyme. LuxS is part of the activated methyl cycle and could also affect biofilm development by AI-2-independent effects on metabolism. A luxS deletion mutant of E. coli W3110 and an inducible plasmid-luxS-complemented strain were used to identify AI-2-independent phenotypes. Differential interference contrast microscopy revealed distinct surface colonization patterns. Confocal microscopy followed by quantitative image analysis determined differences in biofilm topography correlating with luxS expression; deletion mutant biofilms had a 'spreading' phenotype, whereas the complement had a 'climbing' phenotype. Addition of exogenous 4,5-dihydroxy-2,3-pentanedione (DPD), an AI-2 precursor, to the deletion mutant increased biofilm height and biomass, whereas addition of the methyl donor S-adenosyl methionine or aspartate prevented the luxS-complemented strain from producing a thick biofilm. The luxS-complemented strain autoaggregated, indicating that fimbriae production was inhibited, which was confirmed by transmission electron microscopy. DPD could not induce autoaggregation in the deletion mutant, demonstrating that fi...Continue Reading

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Citations

Nov 2, 2016·Environmental Science & Technology·Xiang XiaoHan-Qing Yu
Nov 3, 2016·Frontiers in Microbiology·Laura M CoughlanAvelino Alvarez-Ordóñez
Mar 30, 2017·Infection and Immunity·Kourtney P NickersonChristina S Faherty
Nov 11, 2017·Microbiology·Sneha JaniMichael D Manson
Sep 5, 2019·Frontiers in Microbiology·Carlos E Torres-CernaEsteban A Hernandez-Vargas
Feb 26, 2019·Frontiers in Cellular and Infection Microbiology·Osmel Fleitas MartínezOctavio Luiz Franco
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Mar 28, 2020·Applied Microbiology and Biotechnology·Amandeep KaurPrince Sharma
Oct 11, 2019·Journal of Proteome Research·Gao-Fei DuXuesong Sun

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