Lycopene improves methotrexate-induced functional alterations of the Madin-Darby kidney cells in a concentration-dependent manner

Canadian Journal of Physiology and Pharmacology
Nenad StojiljkovicMilan Stoiljković

Abstract

Lycopene is one of the most potent antioxidants among carotenoids due to its ability to quench singlet oxygen and react with free radicals to reduce DNA damage. Methotrexate is widely used in the treatment of several types of cancers and autoimmune diseases. One of the most common side effects of a high-dose of methotrexate is kidney injury. In this study, we evaluated effects of lycopene on the Madin-Darby canine kidney cells (MDCK) treated with methotrexate through the estimation of their mitochondrial and lysosomal functions ((4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide reduction assay and neutral red uptake assay) and changes in cell oxidative status (determination of advanced oxidized proteins concentrations and reduced glutathione levels) and lysosomal enzymes activity (β-N-acetyl glucosaminidase activity). Results of our study showed that lycopene applied in high concentration caused significant impairment of the MDCK function leading to cell death. Contrarily, in relatively low concentrations lycopene moderately ameliorated methotrexate-induced MDCK cell death estimated by both biochemical and microscopic analyses. It also prevented a significant decline in the MDCK cell lysosomal function estimated by...Continue Reading

References

May 15, 1982·Biochemical Pharmacology·A Leszczyńska, E Pfaff
May 1, 1996·Kidney International·V Witko-SarsatB Descamps-Latscha
Oct 7, 2000·Journal of the American College of Nutrition·A V Rao, S Agarwal
Jul 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tadashi SuzukiYasufumi Emori
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jul 19, 2005·Pediatric Blood & Cancer·Marika GrönroosGianni Celsi
Jun 24, 2006·The Oncologist·Brigitte C Widemann, Peter C Adamson
Oct 3, 2006·Advances in Food and Nutrition Research·A V RaoL G Rao
Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Vishal S VaidyaJoseph V Bonventre
Nov 6, 2008·Archives of Biochemistry and Biophysics·John W ErdmanBrian L Lindshield
Jul 1, 2010·Cell Biochemistry and Function·Premila AbrahamSuganthy Rabi
Dec 9, 2010·Journal of Medicinal Food·José Luis Guil-GuerreroCristóbal López-Sánchez
Jan 1, 2010·Annual Review of Food Science and Technology·Erica N StoryG Keith Harris
Aug 24, 2012·International Journal of Toxicology·Selvinaz DalakliogluArda Tasatargil
Jan 12, 2013·Histology and Histopathology·Yildiz ÇağlarSait Polat
Dec 24, 2013·Advances in Chronic Kidney Disease·Anushree C Shirali, Mark A Perazella
Feb 24, 2015·Journal of Molecular Biology·Jan-Ulrik DahlUrsula Jakob
Sep 1, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Elif OguzNurten Aksoy
Aug 9, 2016·The Oncologist·Scott C HowardR Donald Harvey
Sep 30, 2016·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Nazish Iqbal KhanTabassum Mahboob
Feb 12, 2017·Oncotarget·Benny Zhitomirsky, Yehuda G Assaraf
May 5, 2018·Oxidative Medicine and Cellular Longevity·Nenad StojiljkovicGordana Kocic

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