Lycopene protects against myocardial ischemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening

Drug Design, Development and Therapy
Xuying LiDalin Jia

Abstract

Mitochondria permeability transition pore (MPTP) is an important therapeutic target for myocardial ischemia-reperfusion injury (MIRI). Lycopene (LP) is a potent antioxidant extracted from the mature fruits of plants and has been reported to protect against MIRI; however, its mechanism of action has yet to be completely elucidated. The present study aimed to investigate the role of MPTP in the cardioprotection of LP. H9c2 cells were pretreated with LP for 12 hrs and were subjected to 12-hr hypoxia/1-hr re-oxygenation, and cell viability was measured by a Cell Counting Kit-8 (CCK-8) assay. Male rats were subsequently intraperitoneally injected with LP for 5 consecutive days. At 24 hrs following the final injection, the rat hearts were isolated and subjected to 30-min ischemia/120-min reperfusion using Langendorff apparatus. The myocardial infarct size was measured by a TTC stain. Opening of the MPTP was induced by CaCl2 and measured by colorimetry. The change in mitochondrial transmembrane potential (ΔΨm) was observed under a fluorescence microscope. Apoptosis was measured by flow cytometry and a TUNEL stain, and the expression of apoptosis-related proteins was detected by Western blotting. LP pretreatment significantly increased...Continue Reading

Citations

Mar 4, 2020·International Journal of Molecular Sciences·Muntasir BillahRavinay Bhindi
Jul 7, 2020·International Journal of Molecular Medicine·Lingxiao ZhangTongfeng Zhao
Jun 1, 2021·Oxidative Medicine and Cellular Longevity·Mi XiangXiangning Cui
Jul 3, 2021·Nutrients·Ahmed IsmaeelPanagiotis Koutakis
Jul 20, 2021·Journal of Oral Biosciences·Keita KagawaTakuro Sanuki

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Methods Mentioned

BETA
coronary artery bypass
Assay
Protein Assay
flow cytometry

Software Mentioned

Image J
SPSS
Image J2x

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Apoptosis

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