Lycorine exerts antitumor activity against osteosarcoma cells in vitro and in vivo xenograft model through the JAK2/STAT3 pathway

OncoTargets and Therapy
Hongzhi HuZengwu Shao

Abstract

Background: Lycorine, a natural alkaloid, has been indicated to have various physiological effects, including a potential effect against cancer. However, the anticancer effect of lycorine on osteosarcoma (OS) and the detailed molecular mechanisms involved remain unclear. Purpose: The purpose of this study was to examine the effect of lycorine on human OS and elucidated it underlying mechanisms Materials and methods: In vitro assays, OS cells were treated with lycorine at various concentrations. Then the cell proliferation, colony formation, cell cycle distribution, apoptosis, migration and invasion were assayed to detect the anticancer effect of lycorine on OS cell lines. Western blotting analysis was used to verify the expression of related proteins. In addition, the mouse xenograft model was performed to evaluate lycorine's therapeutic potential on OS in vivo. Results: The in vitro results demonstrated that lycorine induced apoptosis and cell cycle arrest and suppressed the migration and invasion by suppressing constitutive signal transducers and activators of transcription 3 (STAT3) activation through enhancing the expression of SH2 domain-containing phosphatase 1 (SHP-1) and downregulating the expression of STAT3 target pro...Continue Reading

Citations

Jan 16, 2020·Antioxidants·Ana Carolina RuberteCarmen Sanmartín
Apr 8, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Sicong LiLei Bi

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Methods Mentioned

BETA
surgical resection
fluorescence microscopy
flow cytometry
Protein Assay
electrophoresis
immunoprecipitation
Assay
xenograft

Software Mentioned

GraphPad Prism

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