Lycorine inhibits glioblastoma multiforme growth through EGFR suppression

Journal of Experimental & Clinical Cancer Research : CR
Jia ShenMeichun Hu

Abstract

Lycorine has been revealed to inhibit the development of many kinds of malignant tumors, including glioblastoma multiforme (GBM). Although compelling evidences demonstrated Lycorine's inhibition on cancers through some peripheral mechanism, in-depth mechanism studies of Lycotine's anti-GBM effects still call for further exploration. Epidermal Growth Factor Receptor (EGFR) gene amplification and mutations are the most common oncogenic events in GBM. Targeting EGFR by small molecular inhibitors is a rational strategy for GBM treatment. The molecular docking modeling and in vitro EGFR kinase activity system were employed to identify the potential inhibitory effects of Lycorine on EGFR. And the Biacore assay was used to confirm the direct binding status between Lycorine and the intracellular EGFR (696-1022) domain. In vitro assays were conducted to test the suppression of Lycorine on the biological behavior of GBM cells. By RNA interference, EGFR expression was reduced then cells underwent proliferation assay to investigate whether Lycorine's inhibition on GBM cells was EGFR-dependent or not. RT-PCR and western blotting analysis were carried out to investigate the underlined molecular mechanism that Lycorine exerted on EGFR itself ...Continue Reading

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Citations

Jul 31, 2020·Medicinal Research Reviews·Xiao-Fei ShangKuo-Hsiung Lee
Apr 12, 2019·Frontiers in Molecular Neuroscience·Weiyang LouWeimin Fan
Jan 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Guoming ChenHua Xu
Aug 11, 2021·Virologica Sinica·Na LiHan-Qing Ye
Oct 1, 2021·The Protein Journal·Preantha PoonanMahmoud E S Soliman

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Methods Mentioned

BETA
X-ray
xenografts
surface plasmon resonance
chip
PCR
xenografe
fluorescence microscopy
xenograft
nuclear translocation

Software Mentioned

living image
Biacore
AutoDock Toolkit ( ADT )
GraphPad Prism
AutoDock Toolkit
Chemoffice

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