Lycotoxins, antimicrobial peptides from venom of the wolf spider Lycosa carolinensis.

The Journal of Biological Chemistry
L Yan, M E Adams

Abstract

Two peptide toxins with antimicrobial activity, lycotoxins I and II, were identified from venom of the wolf spider Lycosa carolinensis (Araneae: Lycosidae) by virtue of their abilities to reduce ion and voltage gradients across membranes. Both peptides were purified to homogeneity by reversed-phase liquid chromatography and determined to have the following primary structures by Edman microsequencing: IWLTALKFLGKHAAKHLAKQQLSKL-NH2 for lycotoxin I and KIKWFKTMKSIAKFIAKEQMKKHLGGE-OH for lycotoxin II. The predicted secondary structures of the lycotoxins display amphipathic alpha-helix character typical of antimicrobial pore-forming peptides. Antimicrobial assays showed that both lycotoxins potently inhibit the growth of bacteria (Escherichia coli) and yeast (Candida glabrata) at micromolar concentrations. To verify its hypothesized pore-forming activity, lycotoxin I was synthesized and shown to promote efflux of Ca2+ from synaptosomes, to cause hemolysis of erythrocytes, and to dissipate voltage gradients across muscle membrane. The lycotoxins may play a dual role in spider-prey interaction, functioning both in the prey capture strategy as well as to protect the spider from potentially infectious organisms arising from prey ingesti...Continue Reading

References

Aug 3, 1992·European Journal of Pharmacology·R A CrucianiE F Stanley
Oct 1, 1992·The American Journal of the Medical Sciences·J M Futrell
Jun 1, 1992·Antimicrobial Agents and Chemotherapy·F L Schuster, L S Jacob
Nov 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·J W DalyC W Myers
Dec 22, 1992·Biochemistry·S E Blondelle, R A Houghten
Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Aug 1, 1991·Journal of Neurophysiology·V P BindokasM E Adams
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A CrucianiO Colamonici
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·L A RibeiroS de A Nishioka
Jan 1, 1989·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·M E AdamsV J Venema
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff
Aug 29, 1988·FEBS Letters·H C ChenC M Huang
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Jan 1, 1995·Molecular Plant-microbe Interactions : MPMI·A G Rao
Oct 1, 1994·Pflügers Archiv : European journal of physiology·G M NicholsonT Narahashi
Jun 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D S ReillyM Zasloff
Jan 1, 1995·Annual Review of Microbiology·P Nicolas, A Mor
Sep 20, 1996·The Journal of Biological Chemistry·B A DavletovY A Ushkaryov
Oct 23, 1996·Biochemical and Biophysical Research Communications·V G KrasnoperovA G Petrenko

❮ Previous
Next ❯

Citations

May 20, 1999·Biopolymers·D Andreu, L Rivas
Aug 10, 2000·Biopolymers·A TossiA Giangaspero
Oct 12, 2012·World Journal of Microbiology & Biotechnology·Mingfu NiuPuyan Chen
Apr 7, 2004·Toxicon : Official Journal of the International Society on Toxinology·Lucia Kuhn-NentwigWolfgang Nentwig
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·G J Binford
Nov 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Lachlan D Rash, Wayne C Hodgson
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Cyril GoudetJan Tytgat
Feb 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·S HaeberliW Nentwig
Aug 10, 2000·European Journal of Biochemistry·A Torres-LariosL D Possani
Dec 19, 2003·Nucleic Acids Research·M BrahmacharyV B Bajic
Feb 9, 2011·Antimicrobial Agents and Chemotherapy·Marisa Ann AzadCynthia Ross Friedman
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·Zh I Andreeva-KovalevskayaV I Ternovsky
Mar 10, 2010·Biochemistry. Biokhimii︠a︡·A A VassilevskiE V Grishin
Feb 9, 2010·Bioscience, Biotechnology, and Biochemistry·Masahiro MiyashitaHisashi Miyagawa
Nov 10, 2011·Toxins·Natalie J SaezGlenn F King
Aug 25, 2001·Biochemical and Biophysical Research Communications·L DaiT Nakajima
Feb 13, 2014·The Journal of Antibiotics·Nidhi Singh, Jayanthi Abraham
Jul 6, 2000·Bioscience, Biotechnology, and Biochemistry·S MachidaY Yu
Jun 24, 2014·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Jafar JalaeiSeyed Shahram Shekarforoush
Apr 29, 2014·Biotechnology Letters·Eric T JohnsonStephen R Hughes
Nov 15, 2011·Toxicon : Official Journal of the International Society on Toxinology·Yves TerratFrédéric Ducancel
Jul 19, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Marisa RangelJosé Carlos De Freitas
Dec 23, 2008·Toxicon : Official Journal of the International Society on Toxinology·P CiscottoC Chávez-Olórtegui
Dec 9, 2014·Biochimica Et Biophysica Acta·Masayoshi OkadaLourival D Possani
Mar 1, 2017·Toxicon : Official Journal of the International Society on Toxinology·Filippo FratiniAntonio Felicioli
Mar 30, 2001·The Journal of Biological Chemistry·J OrivelJ Rossier
Jun 26, 2003·Protein Science : a Publication of the Protein Society·Pierre EscoubasHervé Darbon
May 4, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Hongwen ZhaoHaining Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.