LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha

Amino Acids
D M SantosM E de Lima

Abstract

LyeTx I, an antimicrobial peptide isolated from the venom of Lycosa erythrognatha, known as wolf spider, has been synthesised and its structural profile studied by using the CD and NMR techniques. LyeTx I has shown to be active against bacteria (Escherichia coli and Staphylococcus aureus) and fungi (Candida krusei and Cryptococcus neoformans) and able to alter the permeabilisation of L: -alpha-phosphatidylcholine-liposomes (POPC) in a dose-dependent manner. In POPC containing cholesterol or ergosterol, permeabilisation has either decreased about five times or remained unchanged, respectively. These results, along with the observed low haemolytic activity, indicated that antimicrobial membranes, rather than vertebrate membranes seem to be the preferential targets. However, the complexity of biological membranes compared to liposomes must be taken in account. Besides, other membrane components, such as proteins and even specific lipids, cannot be discarded to be important to the preferential action of the LyeTx I to the tested microorganisms. The secondary structure of LyeTx I shows a small random-coil region at the N-terminus followed by an alpha-helix that reached the amidated C-terminus, which might favour the peptide-membrane...Continue Reading

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Citations

Mar 21, 2013·Journal of Periodontology·Jessika ConsuegraMaria Esperanza Cortés
Aug 25, 2010·Amino Acids·Mario Sergio Palma
Aug 20, 2015·Cellular and Molecular Life Sciences : CMLS·Peter V DubovskiiRoman G Efremov
Apr 26, 2016·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Leonardo Lima FuscaldiValbert Nascimento Cardoso
Oct 30, 2016·Toxicon : Official Journal of the International Society on Toxinology·Felipe Roberto Borba FerreiraPatrícia Maria Guedes Paiva
Jan 18, 2020·Current Protein & Peptide Science·Marcella Nunes Melo-BragaMaria Elena de Lima
Aug 10, 2018·Toxicology Research·Hassan M Akef
Dec 20, 2018·Toxins·Paula J Segura-Ramírez, Pedro I Silva Júnior
Nov 28, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Flavia Rodrigues da SilvaArmando da Silva-Cunha
Nov 19, 2018·Amino Acids·Mostafa A L Abdel-SalamElaine Maria de Souza-Fagundes
Nov 12, 2020·Experimental Biology and Medicine·Leonardo Lima FuscaldiMaria Elena de Lima
Mar 18, 2021·Bioscience, Biotechnology, and Biochemistry·Alhussin M A MegalyHisashi Miyagawa
Aug 29, 2021·Biological Reviews of the Cambridge Philosophical Society·Tim LüddeckeAndreas Vilcinskas

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