Lymph Drainage from the Chylocyst-Induced Hemodilution in an In Vivo Rabbit Study

Lymphatic Research and Biology
Kumiko AjimaToshio Ohhashi

Abstract

To confirm our previous study that abdominal respiration has induced hemodilution in human subjects, we performed in-vivo experiments involving anesthetized rabbits. Fifteen 6- to 7-month-old male Japanese white rabbits were used in the animal experiments. Anesthesia was maintained with 2.5%-3.0% isoflurane under N2O + 100% O2 inhalation. Ventilation was maintained at 40 mL/breath for 20 breaths/min. Physiological saline solution was administered at rated 18 mL/h during the experiments. First, we attempted to evaluate lymph flow through the thoracic duct using Sonazoid-based contrast-enhanced ultrasound (CEUS)-guided method and then investigated the effects of manual lymph drainage of the chylocyst on the numbers of red blood cells (RBC), hematocrit (Ht) levels, and the blood concentrations of total protein (TP) and hemoglobin (Hb). In this study, we established surgical methods for identifying the left venous angle and chylocyst using Evans blue dye in anesthetized rabbits. We also confirmed that a Sonazoid-based CEUS-guided method was the most useful technique for producing real-time images of lymph flow through the thoracic duct in anesthetized rabbits. In addition, in present experiments involving anesthetized rabbits, we c...Continue Reading

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Aug 26, 2015·Lymphatic Research and Biology·Yoshiko KawaiToshio Ohhashi

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Citations

Jan 7, 2020·Lymphatic Research and Biology·Moyuru HayashiToshio Ohhashi

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Methods Mentioned

BETA
sedation

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