Lymph Node-Targeting Nanovaccine through Antigen-CpG Self-Assembly Potentiates Cytotoxic T Cell Activation

Journal of Immunology Research
Xiaobo XiGuanghui Ma

Abstract

Therapeutic vaccines that arouse the cytotoxic T cell immune response to reject infected cells have been investigated extensively for treating disease. Due to the large amounts of resident antigen-presenting cells (APCs) and T cells in lymph nodes, great efforts have been made to explore the strategy of targeting lymph nodes directly with nanovaccines to activate T cells. However, these nanovaccines still have several problems, such as a low loading efficiency and compromised activity of antigens and adjuvants derived from traditional complicated preparation. There are also safety concerns about materials synthesized without FDA approval. Herein, we construct an assembled nanoparticle composed of an antigen (ovalbumin, OVA) and adjuvant (CpG) to ensure its safety and high loading efficiency. The activity of both components was well preserved due to the mild self-assembly process. The small size, narrow distribution, negative charge, and good stability of the nanoparticle endow these nanovaccines with superior capacity for lymph node targeting. Correspondingly, the accumulation at lymph nodes can be improved by 10-fold. Subsequently, due to the sufficient APC internalization and maturation in lymph nodes, ~60% of T cells are sti...Continue Reading

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Citations

Feb 14, 2021·Biomaterials·Fangmin ChenHaijun Yu
Aug 5, 2020·Advanced Drug Delivery Reviews·Alexander J Najibi, David J Mooney
Sep 19, 2020·Accounts of Chemical Research·Yufei XiaGuanghui Ma
Sep 14, 2021·Frontiers in Immunology·Gregory P HowardRhoel R Dinglasan

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Methods Mentioned

BETA
protein assay
scanning electron microscopy
circular dichroism
fluorescence imaging

Software Mentioned

FlowJo

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