Lymphatic endothelial cell calcium pulses are sensitive to spatial gradients in wall shear stress

Molecular Biology of the Cell
Vinay N SuryaAlexander R Dunn

Abstract

Cytosolic calcium (Ca2+) is a ubiquitous second messenger that influences numerous aspects of cellular function. In many cell types, cytosolic Ca2+ concentrations are characterized by periodic pulses, whose dynamics can influence downstream signal transduction. Here, we examine the general question of how cells use Ca2+ pulses to encode input stimuli in the context of the response of lymphatic endothelial cells (LECs) to fluid flow. Previous work shows that fluid flow regulates Ca2+ dynamics in LECs and that Ca2+-dependent signaling plays a key role in regulating lymphatic valve formation during embryonic development. However, how fluid flow might influence the Ca2+ pulse dynamics of individual LECs has remained, to our knowledge, little explored. We used live-cell imaging to characterize Ca2+ pulse dynamics in LECs exposed to fluid flow in an in vitro flow device that generates spatial gradients in wall shear stress (WSS), such as are found at sites of valve formation. We found that the frequency of Ca2+ pulses was sensitive to the magnitude of WSS, while the duration of individual Ca2+ pulses increased in the presence of spatial gradients in WSS. These observations provide an example of how cells can separately modulate Ca2+ ...Continue Reading

References

Jul 1, 1992·Pflügers Archiv : European journal of physiology·G SchwarzB Nilius
Feb 11, 1992·The American Journal of Physiology·J ShenM A Gimbrone
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J AndoA Kamiya
Sep 1, 1996·Journal of Vascular Research·G HelmlingerR M Nerem
Nov 30, 2000·The Journal of Biological Chemistry·G R Crabtree
Jun 13, 2006·The Journal of Biological Chemistry·Jonathan SoboloffDonald L Gill
Sep 23, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·J Brandon DixonDavid C Zawieja
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mirianas ChachisvilisJohn A Frangos
Dec 18, 2007·Cell·David E Clapham
Feb 24, 2009·Mechanisms of Development·Rishikesh M KulkarniAnn L Akeson
Apr 29, 2009·The Journal of Cell Biology·Camilla NorrménTatiana V Petrova
Dec 12, 2012·Molecular Cell·Nissan YissacharNir Friedman
May 15, 2013·Cellular and Molecular Life Sciences : CMLS·Lara Planas-Paz, Eckhard Lammert
Nov 26, 2013·Biochimica Et Biophysica Acta·Erik Smedler, Per Uhlén
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sanjeev S RanadeArdem Patapoutian
Jul 16, 2014·Microvascular Research·Daniel Vittet
Jan 27, 2015·American Journal of Physiology. Heart and Circulatory Physiology·M JafarnejadJ E Moore
Mar 25, 2015·The Journal of Cell Biology·Brian G CoonMartin A Schwartz
Jul 28, 2015·The Journal of Clinical Investigation·Daniel T SweetMark L Kahn
Sep 4, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jeffrey A KornutaJ Brandon Dixon
Sep 22, 2015·The Journal of Clinical Investigation·Amélie SabineTatiana V Petrova
Mar 7, 2017·The Journal of Clinical Investigation·Dongwon ChoiYoung-Kwon Hong
Apr 21, 2018·Cell·Jie XuArdem Patapoutian
Jun 30, 2018·The Journal of Biological Chemistry·Xiangyu CaiDonald L Gill

❮ Previous
Next ❯

Citations

Mar 25, 2021·Mathematical Biosciences and Engineering : MBE·Christopher D BertramCharlie Macaskill
Jun 27, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tevin C Y ChauBenjamin M Hogan

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Software Mentioned

Fiji ImageJ
StackReg
MATLAB

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.