Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function.

Experimental Cell Research
Jay W ShinMichael Detmar

Abstract

Lymphatic vessels play an important role in the maintenance of tissue fluid homeostasis and in the transport of immune cells to lymph nodes, but they also serve as the major conduit for cancer metastasis to regional lymph nodes. However, the molecular mechanisms regulating these functions are poorly understood. Based on transcriptional profiling studies of cultured human dermal lymphatic (LEC) versus blood vascular endothelial cells (BEC), we found that dipeptidyl peptidase IV (DPPIV) mRNA and protein are much more strongly expressed by cultured lymphatic endothelium than by blood vascular endothelium that only expressed low levels of DPPIV in culture. The enzymatic cleavage activity of DPPIV was significantly higher in cultured LEC than in BEC. Differential immunofluorescence analyses of human organ tissue microarrays for DPPIV and several vascular lineage-specific markers revealed that DPPIV is also specifically expressed in situ by lymphatic vessels of the skin, esophagus, small intestine, breast and ovary. Moreover, siRNA-mediated DPPIV knockdown inhibited LEC adhesion to collagen type I and to fibronectin, and also reduced cell migration and formation of tube-like structures. These results identify DPPIV as a novel lymphat...Continue Reading

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Citations

Oct 8, 2013·Human Reproduction Update·H M Brown, D L Russell
Nov 11, 2011·Cell Biology International·Peiliang Wang, Yongbo Cheng
Jul 11, 2016·Insect Biochemistry and Molecular Biology·Valeriia F TereshchenkovaElena N Elpidina
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Sep 22, 2017·Advances in Bioinformatics·Lina RozanoIndu Bala Jaganath
Aug 14, 2019·Journal of Pharmaceutical Sciences·Ninad VarkhedeM Laird Forrest

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