Abstract
Analyses of microvascular networks with traditional tracer filling techniques suggest that the blood and lymphatic systems are distinct without direct communications, yet involvement of common growth factors during angiogenesis and lymphangiogenesis suggest that interactions at the capillary level are possible. To investigate the structural basis for lymphatic/blood endothelial cell connections during normal physiological growth, the objective of this study was to characterize the spatial relations between lymphatic and blood capillaries in adult rat mesenteric tissue. Using immunohistochemical methods, adult male Wistar rat mesenteric tissues were labeled with antibodies against PECAM (an endothelial marker) and LYVE-1, Prox-1, or Podoplanin (lymphatic endothelial markers) or NG2 (a pericyte marker). Positive PECAM labeling identified apparent lymphatic/blood endothelial cell connections at the capillary level characterized by direct contact or direct alignment with one another. In PECAM labeled networks, a subset of the lymphatic and blood capillary blind ends were connected with each other. Intravital imaging of FITC-Albumin injected through the femoral vein did not identify lymphatic vessels. At contact sites, lymphatic end...Continue Reading
References
Nov 1, 1977·Microvascular Research·G W Schmid-SchoenbeinS Kovalcheck
Mar 1, 1988·The American Journal of Physiology·J L Unthank, H G Bohlen
Jan 1, 1986·Journal of Microscopy·T Gahm, S Witte
Mar 30, 1973·Pflügers Archiv : European journal of physiology·G Hauck
Jul 1, 1984·Microvascular Research·T C SkalakB W Zweifach
Aug 3, 2002·Nature Reviews. Cancer·Steven A StackerKari Alitalo
Jan 11, 2003·Science·Farhad AbtahianMark L Kahn
Jan 22, 2003·Microvascular Research·Ana M Ponce, Richard J Price
Sep 27, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Christopher R AndersonRichard J Price
Jul 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Christopher R AndersonRichard J Price
Apr 13, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter L MurfeeShayn M Peirce
Sep 3, 2005·The Journal of Clinical Investigation·Kazuichi MaruyamaJ Wayne Streilein
Dec 14, 2005·Experimental Cell Research·Terhi Karpanen, Taija Mäkinen
Apr 22, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Walter L MurfeeThomas C Skalak
Jan 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Sep 6, 2008·The American Journal of Pathology·Christopher R AndersonRichard J Price
Dec 6, 2008·Genes & Development·Nicole C JohnsonGuillermo Oliver
Feb 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Souheil El-ChemalyJoel Moss
Citations
Dec 18, 2012·Lymphatic Research and Biology·Richard S SweatWalter L Murfee
Nov 6, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Peter C StaporWalter L Murfee
Jun 23, 2012·BMC Physiology·Molly R Kelly-GossWalter L Murfee
May 31, 2012·Journal of Visualized Experiments : JoVE·Ming YangWalter L Murfee
Nov 29, 2015·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Bruce A CorlissWalter Lee Murfee
Jun 15, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Lasse Dahl JensenYihai Cao
Mar 25, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Richard S SweatWalter L Murfee
Jul 13, 2016·Physiological Reports·Richard S SweatWalter L Murfee
Jul 21, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Theresa B PhamduyDouglas B Chrisey
May 21, 2018·Journal of Vascular Research·Ariana D Suarez-MartinezWalter L Murfee
Mar 17, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yang LeeMariappan Muthuchamy
Apr 20, 2011·Lab on a Chip·Omid ForouzanSergey S Shevkoplyas
Oct 5, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Mohammad S AzimiWalter L Murfee
Apr 8, 2020·ACS Pharmacology & Translational Science·Lauren F Sestito, Susan N Thomas