Lymphocyte apoptosis in the inflammatory reaction around Taenia solium metacestodes in porcine cysticercosis

Veterinary Parasitology
Sandra SolanoJ L Molinari

Abstract

In the current research, we report apoptosis of lymphocytes in the inflammatory reaction around metacestodes in muscle tissue from cysticercotic pigs. Two events, high metacestode viability (100%) and high cysteine protease activity were found to be closely related to a high phosphatydilserine expression by inflammatory lymphocytes (56%). Testing the RPMI medium used for washing away inflammatory cells from metacestodes with 100% viability, with the fluorescent substrate Z-Phe-Ala-AFC for measuring cysteine protease activity, significant fluorescent values were found. In contrast, tests performed with RPMI medium used for washing away inflammatory cells from metacestodes with 90% viability or less, showed low fluorescence values. Flow cytometry analyses of inflammatory cells obtained from four naturally cysticercotic pigs, and stained with Annexin-V/PI, showed lymphocytes expressing phosphatidylserine with values of 0, 6, 41 and 56% on their outer surfaces. Electron microscopy studies of inflammatory cells from metacestodes with 100% viability, showed lymphocytes with strangled and fragmented nuclei, and heterochromatin displaced to the nuclear periphery. In addition, DNA from these cells showed fragmentation in electrophoresis...Continue Reading

Citations

Mar 14, 2013·BioMed Research International·Alberto N PeónLuis I Terrazas
Oct 27, 2010·Expert Review of Proteomics·Young-An BaeYoon Kong
Dec 3, 2011·Veterinary Parasitology·Luz Adilia Luna-OlivaresTine Moesgaard Iburg
Mar 9, 2018·Parasite Immunology·Á DíazC Casaravilla

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis