Lymphocyte CFTR promotes epithelial bicarbonate secretion for bacterial killing

Journal of Cellular Physiology
Xiao Xiao TangHsiao Chang Chan

Abstract

The expression of cystic fibrosis transmembrane conductance regulator (CFTR) in lymphocytes has been reported for nearly two decades; however, its physiological role remains elusive. Here, we report that co-culture of lymphocytes with lung epithelial cell line, Calu-3, promotes epithelial HCO(3)- production/secretion with up-regulated expression of carbonic anhydrase 2 and 4 (CA-2, CA-4) and enhanced bacterial killing capability. The lymphocyte-enhanced epithelial HCO(3)- secretion and bacterial killing activity was abolished when Calu3 cells were co-cultured with lymphocytes from CFTR knockout mice, or significantly reduced by interfering with E-cadherin, a putative binding partner of CFTR. Bacterial lipopolysaccharide (LPS)-induced E-cadherin and CA-4 expression in the challenged lung was also found to be impaired in CFTR knockout mice compared to that of the wild-type. These results suggest that the interaction between lymphocytes and epithelial cells may induce a previously unsuspected innate host defense mechanism against bacterial infection by stimulating epithelial HCO(3)- production/secretion, which requires CFTR expression in lymphocytes.

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Citations

Sep 29, 2015·The Journal of Thoracic and Cardiovascular Surgery·Don HayesHeidi M Mansour
Feb 25, 2014·The International Journal of Biochemistry & Cell Biology·Pauline T IkpaHugo R de Jonge
Jun 15, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Don HayesDmitry Tumin
Feb 6, 2017·American Journal of Respiratory and Critical Care Medicine·Yong HuangUNKNOWN COMET-IPF Investigators
Oct 5, 2018·Frontiers in Microbiology·Orsolya DobayÁkos Zsembery
Apr 26, 2019·Scientific Reports·Juliette SimoninIsabelle Sermet-Gaudelus
Apr 4, 2021·International Journal of Molecular Sciences·Miroslaw ZajacIsabelle Sermet-Gaudelus

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