PMID: 2145240Jan 1, 1990Paper

Lymphocyte electrophoresis as an indicator of modulation of concanavalin A-induced suppressor T-cells in vivo by anti-cancer drugs

International Journal of Immunopharmacology
M ShimizuT Iwaguchi


We have previously reported that concanavalin A (Con A)-induced suppressor T-cells in vivo are located in the lower electrophoretic mobility (EPM) zone as judged by the ratio of low mobility T-cells to high mobility T-cells (LMT/HMT ratio) in terms of lymphocyte electrophoresis, and that the administration of 5-fluorouracil (5-FU) eliminates their suppressor activity as assayed by Con A-induced lymphocyte blastogenesis in vitro. The current study was undertaken to clarify the relation between changes in EPM and suppressor activity of Con A-induced suppressor T-cells by the administration of antitumor drugs such as cyclophosphamide (CY) and 5-FU when assayed by production of anti-sheep red blood cell antibody in vitro. The pretreatment with 5-FU, but not CY, abrogated the suppressor activity of Con A-induced T-cells followed by restoration of the LMT/HMT ratio of Con A-induced T-cells to that of normal T-cells. These finding suggest that lymphocyte electrophoresis can be applied to an assay for screening immunomodulating agents in Con A-induced suppressor T-cells.


Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Jan 1, 1975·Immunological Communications·S DavisG R Pearson
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K SugiuraS Ikehara
Aug 1, 1984·Cellular Immunology·S R SmithA S Watnick

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