Lymphocyte modulation with FTY720 improves hemorrhagic shock survival in swine.

PloS One
Jason S HawksworthEric A Elster

Abstract

The inflammatory response to severe traumatic injury results in significant morbidity and mortality. Lymphocytes have recently been identified as critical mediators of the early innate immune response to ischemia-reperfusion injury. Experimental manipulation of lymphocytes following hemorrhagic shock may prevent secondary immunologic injury in surgical and trauma patients. The objective of this study is to evaluate the lymphocyte sequestration agent FTY720 as an immunomodulator following experimental hemorrhagic shock in a swine liver injury model. Yorkshire swine were anesthetized and underwent a grade III liver injury with uncontrolled hemorrhage to induce hemorrhagic shock. Experimental groups were treated with a lymphocyte sequestration agent, FTY720, (n = 9) and compared to a vehicle control group (n = 9). Animals were observed over a 3 day survival period after hemorrhage. Circulating total leukocyte and neutrophil counts were measured. Central lymphocytes were evaluated with mesenteric lymph node and spleen immunohistochemistry (IHC) staining for CD3. Lung tissue infiltrating neutrophils were analyzed with myeloperoxidase (MPO) IHC staining. Relevant immune-related gene expression from liver tissue was quantified using R...Continue Reading

References

Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Aug 3, 1989·The New England Journal of Medicine
Jan 1, 1993·The British Journal of Surgery·A C WindsorH J Sugerman
May 1, 1997·Kidney International·H RabbH R Brady
Jul 15, 1997·The Journal of Clinical Investigation·R M ZwackaJ F Engelhardt
Sep 1, 1997·The Journal of Clinical Investigation·M TakadaN L Tilney
Dec 5, 1998·Seminars in Immunology·P Matzinger
Feb 2, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Y HorieD N Granger
Mar 9, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·S KokuraT Y Aw
May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·O Le MoineJ Devière
Jun 30, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·A K DeC L Miller-Graziano
Sep 1, 2000·American Journal of Physiology. Renal Physiology·H RabbW W Tang
Sep 26, 2001·Transplantation·V BrinkmannS Chen
Nov 7, 2001·The Journal of Clinical Investigation·M J BurneH Rabb
May 11, 2002·Critical Care Medicine·John M Harlan, Robert K Winn
Aug 17, 2002·Current Opinion in Immunology·Volker Brinkmann, Kevin R Lynch
Jan 1, 2004·Nature·Tom Clarke
Feb 12, 2004·Kidney International·Duska DragunHans-Hellmut Neumayer
Jun 16, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Volker BrinkmannTimothy Hla
Aug 17, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yi ZhaoSheung Tat Fan
Nov 19, 2004·Journal of Leukocyte Biology·Karyn Yonekawa, John M Harlan
Feb 15, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Lev KoyrakhKevin Wickman
Jan 27, 2007·Critical Care Medicine·Gautam BandyopadhyayCarol Miller-Graziano
Feb 7, 2007·Current Opinion in Pharmacology·Stephan L M Peters, Astrid E Alewijnse
May 2, 2007·Journal of Leukocyte Biology·Charles C CaldwellAlex B Lentsch
Oct 19, 2007·Cellular Immunology·Yanfei HuangKarl L Womer
Dec 1, 2007·Injury·Andreas LenzWilliam G Cheadle
Jan 1, 2008·Transplantation·Timothy A Weaver, Allan D Kirk
Sep 13, 2008·Nature Reviews. Immunology·Juan RiveraAna Olivera
Aug 7, 2009·Journal of Thrombosis and Haemostasis : JTH·J S HawksworthD K Tadaki
Oct 10, 2009·Physiology·Tobias EckleHolger K Eltzschig
Jan 22, 2010·The New England Journal of Medicine·Ludwig KapposUNKNOWN FREEDOMS Study Group
Jan 22, 2010·The New England Journal of Medicine·Jeffrey A CohenUNKNOWN TRANSFORMS Study Group

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Methods Mentioned

BETA
blood collection

Software Mentioned

SPSS

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