Lymphocyte trafficking: CD4 T cells with a 'memory' phenotype (CD45RC-) freely cross lymph node high endothelial venules in vivo

Immunology
S M Sparshott, E B Bell

Abstract

Antigen encounter not only induces a change in surface expression of CD45RC isoforms in the rat from a high (CD45RC+) to a low molecular weight molecule (CD45RC-), but also stimulates changes in expression of adhesion molecules that regulate CD4 T-cell migration. T cells with an activated or 'memory' phenotype (CD45RC-) are thought to enter lymph nodes almost exclusively via afferent lymphatics whereas T cells in a resting state (CD45RC+) migrate across high endothelial venules (HEV). The present study monitored the rapid recirculation from blood to lymph of allotype-marked CD45RC T-cell subsets. Surprisingly, we found that CD45RC- CD4 T cells entered the thoracic duct slightly faster and reached peak numbers 3 hr earlier (18 hr) than did the CD45RC+ subset. To determine whether the entrance of CD45RC+ and RC- subsets was restricted to HEV and afferent lymphatics, respectively, recirculation of CD4 T cells was monitored in mesenteric lymphadenectomized (MLNx) rats (on healing the intestinal afferent lymphatics are joined directly to the thoracic duct), or in recipients that had had the mesenteric lymph node (MLN) acutely (2-3 hr) deafferentized (entry would be restricted to HEV). In these studies CD45RC- CD4 T cells entered the...Continue Reading

References

Apr 1, 1992·European Journal of Immunology·C R MackayW R Hein
Nov 19, 1992·Nature·C A MichieP C Beverley
Oct 1, 1991·The Journal of Cell Biology·R E MebiusG Kraal
Nov 8, 1990·Nature·E B Bell, S M Sparshott
Mar 1, 1990·The Journal of Experimental Medicine·C R MackayL Dudler
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BirkelandE Puré
Jun 1, 1983·The Journal of Experimental Medicine·C W PughH W Steer
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R Mclean, C A Michie
Nov 1, 1994·European Journal of Immunology·S M Sparshott, E B Bell
Jan 1, 1993·European Journal of Immunology·S R SarawarE B Bell
Jan 28, 1994·Cell·J Sprent
May 1, 1993·Clinical and Experimental Immunology·H J RothkötterR Pabst
Jan 1, 1993·Advances in Immunology·C R Mackay
Nov 1, 1996·International Immunology·D PillingM Salmon
Jun 1, 1996·Immunology Today·J Westermann, R Pabst
Feb 17, 1997·The Journal of Experimental Medicine·C Bunce, E B Bell

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Citations

May 8, 1999·Immunology·D M HaigH R Miller
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F Ramírez, D Mason
Jan 1, 2010·Archives of Histology and Cytology·Kenjiro MatsunoChangde Shi
Feb 26, 2009·International Immunology·Antje KlingerJürgen Westermann
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·C A LondonA K Abbas
Mar 21, 2001·Critical Reviews in Clinical Laboratory Sciences·G WiedleB A Imhof
Aug 6, 2003·Nuclear Medicine and Biology·Alessandro FulgenziMaria Elena Ferrero

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