Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes.

The International Journal of Biochemistry & Cell Biology
Katalin É Szabó-TaylorPaul G Winyard

Abstract

Peroxiredoxin 2 has immune regulatory functions, but its expression in human peripheral blood lymphocytes and levels in extracellular fluid in healthy subjects and rheumatoid arthritis patients are poorly described. In the present study, the median intracellular peroxiredoxin 2 protein content of lymphocytes from rheumatoid arthritis patients was more than two-fold higher compared with healthy subjects' lymphocytes. Intracellular peroxiredoxin 3 levels were similar in healthy and rheumatoid arthritis lymphocytes. Flow cytometry detected peroxiredoxin 2 on the surface of ca. 8% of T cells and ca. 56% of B cells (median % values) of all subjects analyzed. Exofacial thioredoxin-1 was also observed. In the total lymphocyte population from rheumatoid arthritis patients, few cells (median, 6%) displayed surface peroxiredoxin 2. In contrast, a significantly increased proportion of interleukin-17(+ve) lymphocytes were exofacially peroxiredoxin 2(+ve) (median, 39%). Prdx2 was also detected in human extracellular fluids. We suggest that crucial inflammatory cell subsets, i.e. interleukin-17(+ve) T cells, exhibit increased exofacial redox-regulating enzymes and that peroxiredoxin 2 may be involved in the persistence of pro-inflammatory ce...Continue Reading

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Citations

Sep 7, 2013·Chemistry Central Journal·Rita Barreto Duarte Carilho TorraoHelen R Griffiths
Jan 15, 2014·Arthritis Research & Therapy·Sorcha FinneganMadeleine E Rooney
Sep 29, 2015·Antioxidants & Redox Signaling·Jeroen FrijhoffPietro Ghezzi
Sep 18, 2014·Journal of Proteomics·Emilie ViennoisDidier Merlin
Aug 12, 2014·Biochemical Society Transactions·Helen R GriffithsChristopher R Dunston
Feb 28, 2015·Seminars in Cell & Developmental Biology·Katalin Szabó-TaylorEdit I Buzás
Mar 19, 2014·Free Radical Biology & Medicine·Rita C TorrãoHelen R Griffiths
May 1, 2016·Pharmacology & Therapeutics·Mi Hee ParkJin Tae Hong
Jun 28, 2019·Journal of Applied Physiology·Alex J WadleySteven J Coles
Jan 8, 2020·Antioxidants & Redox Signaling·Leonardo Y TanakaFrancisco R M Laurindo
Nov 22, 2016·Journal of Gastroenterology and Hepatology·Nezha SenhajiAbdelaziz Soukri
Apr 18, 2018·Seminars in Immunopathology·Edit I BuzásKatalin É Szabó-Taylor
Oct 28, 2020·Biological Chemistry·Inken LorenzenEva-Maria Hanschmann

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Methods Mentioned

BETA
Assay
flow cytometry

Software Mentioned

Quantity One

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