PMID: 8946685Oct 1, 1996Paper

Lymphocytes subsets in normal individuals: analysis by four color immunofluorescence and flow cytometry on whole blood

Tissue Antigens
V SchiavonL Boumsell

Abstract

In order to precisely define human lymphocytes subsets, we used four color immunofluorescence analysis and flow cytometry. We report here the results of systematic studies performed on whole blood from 20 normal volunteers. This was performed using monoclonal antibodies recognizing 18 different CD molecules and analyzed here into 14 different 4-color immunofluorescence combinations. This showed that among T cells, the two major CD3+CD8+ and CD3+CD8- subsets differed greatly in the percentage of cells expressing CD26, CD27, CD28, CD38, CD45RA, CD45RO, CD57, S6F1, BY55, CD101 and their respective combination representing an array of subsets. Furthermore the minor NK subsets, i.e. CD2+CD3-CD8+, CD2-CD3-CD8+, CD2+CD3-CD8-, CD2-CD3-CD8- also differed in percentages of cells expressing CD16, CD56, CD57, S6F1, BY55 and their combinations.

References

Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A BensussanE Carosella
Mar 1, 1994·International Immunology·C GouttefangeasA Bensussan
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A BensussanL Boumsell

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Citations

Jun 12, 1999·Journal of Immunological Methods·J M FaintM Salmon
Sep 23, 2010·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Howard J Meyerson
Oct 12, 2012·Asian Journal of Psychiatry·Bisu SinghTapas Kumar Chaudhuri
Jun 8, 2001·American Journal of Clinical Pathology·D JonesY O Huh
Dec 18, 1998·The Journal of Investigative Dermatology·M BagotL Boumsell
Feb 9, 2017·Clinical and Experimental Immunology·M S HasanF Fortune
May 1, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J Paul Robinson

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