PMID: 7516402Jul 1, 1994Paper

Lymphoid and myeloid differentiation of fetal liver CD34+lineage- cells in human thymic organ culture

The Journal of Experimental Medicine
A BárcenaH Spits

Abstract

In this article, we report that the human fetal thymus contains CD34bright cells (< 0.01% of total thymocytes) with a phenotype that resembles that of multipotent hematopoietic progenitors in the fetal bone marrow. CD34bright thymocytes were CD33-/dull and were negative for CD38, CD2, and CD5 as well as for the lineage markers CD3, CD4, and CD8 (T cells), CD19 and CD20 (B cells), CD56 (NK cells), glycophorin (erythrocytes), and CD14 (monocytes). In addition, total CD34+ lineage negative (lin-) thymocytes contained a low number of primitive myeloid progenitor cells, thus suggesting that the different hematopoietic lineages present in the thymus may be derived from primitive hematopoietic progenitor cells seeding the thymus. To investigate whether the thymus is permissive for the development of non-T cells, human fetal organ culture (FTOC) assays were performed by microinjecting sorted CD34+lin- fetal liver cells into fragments of HLA-mismatched fetal thymus. Sequential phenotypic analysis of the FTOC-derived progeny of CD34+lin- cells indicated that the differentiation into T cells was preceded by a wave of myeloid differentiation into CD14+CD11b+CD4dull cells. Donor-derived B cells (CD19+CD20+) were also generated, which produc...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M BaumB Peault
Oct 1, 1992·Immunology Today·L L LanierJ H Phillips
Mar 6, 1992·Cell·P MombaertsV E Papaioannou
Dec 1, 1991·The Journal of Experimental Medicine·L WuK Shortman
Jul 1, 1991·The Journal of Experimental Medicine·M C MingariL Moretta
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BárcenaC Martínez-A
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P S Freudenthal, R M Steinman
Oct 1, 1990·The Journal of Experimental Medicine·R NamikawaJ M McCune
Mar 1, 1987·Journal of Clinical Immunology·D F Lobach, B F Haynes
Dec 26, 1987·Lancet·P G IsaacsonB J Addis
Dec 1, 1989·Cellular Immunology·D J IzonA Kelso
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J KurtzbergB F Haynes
Apr 1, 1989·The Journal of Experimental Medicine·G G Frederickson, R S Basch
May 1, 1988·The Journal of Experimental Medicine·G J SpangrudeI L Weissman
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J PèneJ Banchereau
Dec 1, 1993·The Journal of Experimental Medicine·M J SánchezJ H Phillips
May 1, 1993·The Journal of Experimental Medicine·W CraigP M Lansdorp
Oct 1, 1993·The Journal of Experimental Medicine·Y MatsuzakiH Nakauchi

❮ Previous
Next ❯

Citations

May 7, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Hélène LapillonneGeorges Deschênes
Oct 24, 2000·Journal of Immunological Methods·J T EvansJ V Garcia
Oct 6, 1998·Journal of Immunological Methods·G Anderson, E J Jenkinson
May 30, 2001·Experimental Hematology·M C PoznanskyD T Scadden
Apr 21, 1999·Experimental Hematology·T SatoM Ogawa
May 30, 2001·Trends in Immunology·N S TredeL I Zon
Aug 16, 2002·British Journal of Haematology·Isabel BarãoJoão L Ascensão
Sep 15, 2000·Human Gene Therapy·J GatlinJ V Garcia
Mar 15, 1997·AIDS·G N GaultonM Rosenzweig
Dec 1, 1995·British Journal of Haematology·G E TjønnfjordT Egeland
Apr 1, 2014·Cellular and Molecular Life Sciences : CMLS·Joanna HalkiasEllen A Robey
Sep 1, 1994·Transplant Immunology·R Huss, H J Deeg
Aug 31, 2006·Experimental Hematology·Joao T BarataAngelo A Cardoso
Feb 14, 2006·Immunity·Rima HaddadBruno Canque
Jul 11, 2000·Nature Biotechnology·M C PoznanskyD T Scadden
Mar 17, 2004·Cancer Control : Journal of the Moffitt Cancer Center·Marcus O Muench, Alicia Bárcena
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M O Muench, A Bárcena

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.