Lymphokine gene expression related to CD4 T cell subset (CD45R/CDw29) phenotype conversion.

European Journal of Immunology
F BettensW J Pichler

Abstract

In this study, we investigated whether the phenotype-related differentiation of human 2H4+ (CD45R) naive cells to 2H4- (CDw29) memory CD4 cells corresponded to modulation of interleukin (IL) 2, IL 4, interferon (IFN)-gamma and granulocyte-monocyte colony-stimulating factor (GM-CSF) gene expression, a phenomenon which might correlate to the distinct functional activities of naive cells or memory cells. To mimic in vitro CD4 T cell subset differentiation, freshly isolated 2H4+ and 2H4- CD4 cells were stimulated with the anti-CD3 antibody (Leu-4), expanded in IL 2-containing medium and restimulated with Leu-4 after 7 and 13 days. Absence of monocyte-T cell interaction was compensated by adding monocyte supernatant to the culture medium and by cross-linking the anti-CD3 antibodies with goat anti-mouse antibody coated on culture dishes. It has been previously shown that in vitro stimulated 2H4+ cells acquire CDw29 surface antigens. Measurement of lymphokine gene expression by dot-blot hybridization revealed that although stimulated 2H4+ cells proliferated less than stimulated 2H4- cells, and expressed less actin mRNA, they expressed more IL 2 but less IL 4 and GM-CSF than 2H4- cells. No significant difference was observed between th...Continue Reading

References

Jan 1, 1986·Molecular Biology Reports·E W Khandjian
Nov 1, 1988·The Journal of Experimental Medicine·F T RotteveelC J Lucas
Dec 1, 1987·The Journal of Experimental Medicine·C E RuddS F Schlossman
Sep 1, 1988·European Journal of Immunology·J F GauchatB M Stadler
Jul 11, 1983·Nucleic Acids Research·R DevosW Fiers
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Annual Review of Immunology·K A Smith

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Citations

Mar 1, 1991·Immunology Today·C L King, T B Nutman
Nov 1, 1991·American Journal of Respiratory Cell and Molecular Biology·K P MarathiasR L Kradin
Nov 1, 1992·Cytokine·R J ArmitageK H Grabstein
Apr 1, 1997·Journal of the American Academy of Dermatology·E RobinetA Claudy
Jun 1, 1991·Immunology Today·A N AkbarG Janossy
Sep 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·K YamazakiK Hara
Jun 14, 2017·International Journal of Cancer. Journal International Du Cancer·Yi LiaoShiyong Wu
Apr 1, 1992·Clinical and Experimental Immunology·H BeckerK Federlin
Jan 1, 1994·Clinical and Experimental Immunology·J WangF Emmrich
May 1, 1992·European Journal of Immunology·T Van der Pouw-KraanL Aarden
Jan 1, 1995·Clinical and Diagnostic Laboratory Immunology·J E McElhaneyG S Meneilly
Nov 1, 1996·Clinical and Diagnostic Laboratory Immunology·L J KobrynskiS D Douglas

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