Lymphokine production by peripheral blood leucocytes: quantitation of T-cell growth factor activity for assessment of immune response capability

Australian and New Zealand Journal of Medicine
H S Warren, R G Pembrey

Abstract

Mitogen stimulation of as few as 200,000 human peripheral blood leucocytes (PBL) in 0.2 ml volumes produces highly active lymphokine supernatants. The lymphokine activity measured is T-cell growth factor. Under optimum conditions of PHA-P concentration, serum source and concentration, and time, activity is detectable to a dilution of between 1/250 and 1/700 for lymphokine supernatants from PBL of normal donors. Lymphokine supernatants prepared from PBL of donors previously treated with the immunosuppressive regimes of radiotherapy and/or chemotherapy had activities that were between 13% and 66% of normal controls. Lymphokine production as a quantitative measure of immune capability is discussed.

References

Apr 1, 1978·The Australian Journal of Experimental Biology and Medical Science·H S WarrenK J Lafferty
Jan 1, 1979·Scandinavian Journal of Immunology·H S Warren, K J Lafferty
Jan 1, 1977·Immunological Reviews·K J Lafferty, J Woolnough
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Jan 1, 1980·Immunological Reviews·K J LaffertyS J Prowse
Jan 1, 1981·Journal of Immunological Methods·H S Warren, R G Pembrey

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