PMID: 2492434Jan 30, 1989Paper

Lysine and alanine transport in the perfused guinea-pig placenta

Biochimica Et Biophysica Acta
C P Wheeler, D L Yudilevich

Abstract

The characteristics of L-lysine transport were investigated at brush-border (maternal) and basal (fetal) sides of the syncytiotrophoblast in the term guinea-pig placenta artificially perfused either through the umbilical vessels in situ or through both circulations simultaneously. Cellular uptake, efflux and transplacental transfer were determined using a single-circulation paired-tracer dilution technique. Unidirectional L-[3H]lysine uptake (%) (perfusate lysine 50 microM) was high on maternal (M = 87 +/- 1) and fetal (F = 73 +/- 2) sides. L-[3H]Lysine efflux back into the ipsilateral circulation was asymmetrical (F/M ratio = 2.3) and transplacental flux occurred in favour of the fetal circulation. Unidirectional lysine influx kinetics (0.05-8.00 mM) gave Km values of 1.75 +/- 0.70 mM and 0.90 +/- 0.25 mM at maternal and fetal sides, respectively; corresponding Vmax values were 1.95 +/- 0.38 and 0.87 +/- 0.10 mumol.min-1.g-1. At both sides, lysine influx (50 microM) could be inhibited (about 60-80%) by 4 mM L-lysine and L-ornithine and less effectively (about 10-40%) by L-citrulline, L-arginine, D-lysine and L-histidine. At the basal side: (i) lysine influx kinetics were greatly modified in the presence of 10 mM L-alanine (Km ...Continue Reading

References

Apr 1, 1978·Physiological Reviews·F C Battaglia, G Meschia
Nov 1, 1979·The American Journal of Physiology·D L YudilevichH P Leichtweiss
Mar 1, 1976·The American Journal of Physiology·R H EndersC H Smith
Oct 31, 1988·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K P ForsythM P Alpers
Apr 1, 1987·Metabolism: Clinical and Experimental·B StatesS Segal
Jul 10, 1987·Biochimica Et Biophysica Acta·M A BowringS Segal
Nov 1, 1988·The Journal of Physiology·C P Wheeler, D L Yudilevich
Jun 1, 1988·The American Journal of Physiology·J H Sweiry, D L Yudilevich
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J Lerner
Oct 5, 1987·Biochimica Et Biophysica Acta·D B Shennan, C A Boyd
Aug 15, 1986·The Biochemical Journal·M E GanapathyV Ganapathy
Jan 1, 1986·Gynecologic and Obstetric Investigation·M H Carstensen, H P Leichtweiss
Sep 9, 1985·Biochimica Et Biophysica Acta·D L Yudilevich, J H Sweiry
Apr 1, 1985·Journal of Cellular Physiology·F V Sepúlveda, J D Pearson
Jan 1, 1985·The Journal of Membrane Biology·H N Christensen
Dec 1, 1973·The Journal of Physiology·P M Hill, M Young
Apr 1, 1983·Pflügers Archiv : European journal of physiology·B StiegerH Murer
Apr 1, 1983·Pflügers Archiv : European journal of physiology·G CassanoH Murer
Jan 1, 1984·The International Journal of Biochemistry·K Metoki, F A Hommes
May 1, 1982·Pflügers Archiv : European journal of physiology·I Samarzija, E Frömter
Sep 1, 1981·The American Journal of Physiology·B M Eaton, D L Yudilevich
May 1, 1982·Pflügers Archiv : European journal of physiology·I Samarzija, E Frömter

❮ Previous
Next ❯

Citations

Jan 1, 1994·Molecular Aspects of Medicine·G Desoye, E Shafrir
Feb 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·D B Shennan
Feb 15, 2001·Placenta·F C Battaglia, T R Regnault
Jul 10, 1998·The Proceedings of the Nutrition Society·J C MatthewsD A Novak
Jan 20, 1999·European Journal of Clinical Investigation·B M Eaton, S R Sooranna
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·H N JonesH J McArdle
Aug 1, 1991·The American Journal of Physiology·T C FureszC H Smith
Mar 1, 1995·The American Journal of Physiology·T C FureszC H Smith
Jun 1, 1995·The American Journal of Physiology·A J Moe
Sep 1, 1994·The American Journal of Physiology·M S MalandroD A Novak
Sep 1, 1991·The American Journal of Physiology·J H SweiryG E Mann
Jan 29, 1990·Biochimica Et Biophysica Acta·Y Kudo, C A Boyd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.