Lysine degradation in Candida. Characterization and probable role of L-norleucine-leucine, 4-aminobutyrate and delta-aminovalerate:2-oxoglutarate aminotransferases

Biochimie
P A Der Garabedian, J J Vermeersch

Abstract

Three enzymes partially purified that catalyze respectively the transamination of L-norleucine, 4-aminobutyrate and delta-aminovalerate with alpha-ketoglutarate as aminoacceptor were characterized and isolated from L-lysine adapted cell of Candida guilliermondii var. membranaefaciens. The transaminases have a maximum activity in the pH range of 7.8-8.5 and at 55 degrees C, 45 degrees C and 40 degrees C respectively. alpha-Ketoglutarate and to a lesser extent pyridoxal-5'-phosphate were effective protecting agents against rise in temperature. The enzymes exhibit absorption maximum at 280 nm, 330 nm and 410 nm. The fact that L-norleucine-leucine aminotransferase, 4-aminobutyrate aminotransferase and delta-aminovalerate aminotransferase are strongly induced by growing the yeast Candida on L-lysine suggests new hypothetic pathways for the catabolism of L-lysine where the main substrate of each aminotransferase could be an intermediary metabolite.

References

Aug 1, 1976·Journal of Biochemistry·M KisumiI Chibata
Aug 17, 1987·European Journal of Biochemistry·P A Der Garabedian, J J Vermeersch
May 2, 1986·European Journal of Biochemistry·P A Der GarabedianJ J Vermeersch
Apr 4, 1969·Analytical Biochemistry·M S ReitzV W Rodwell
Jul 26, 1965·Biochemical and Biophysical Research Communications·K HigashinoI Lieberman
Aug 1, 1965·Archives of Biochemistry and Biophysics·M Rothstein
Nov 15, 1965·Biochimica Et Biophysica Acta·K Higashino, I Lieberman
Mar 1, 1967·Archives of Biochemistry and Biophysics·G LindahlS Lindstedt
Jan 1, 1980·Annual Review of Biochemistry·E Adams, L Frank
Oct 18, 1952·Nature·A C HULME, W ARTHINGTON
Feb 1, 1953·The Biochemical Journal·R I MORRISON
Nov 1, 1953·Archives of Biochemistry and Biophysics·P H LOWY
Mar 1, 1954·Journal of Bacteriology·T C STADTMAN
May 1, 1954·Journal of Bacteriology·P M DOHNER, B P CARDON
Aug 1, 1961·Journal of Biochemistry·N ITADAN SASAKI
Sep 1, 1964·Biochemistry·S W TANENBAUM, K KANEKO
Nov 8, 1964·Biochimica Et Biophysica Acta·M ROTHSTEIN, J L HART
Feb 1, 1951·Journal of Bacteriology·H C JACKINS, H A BARKER

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Citations

Oct 1, 2003·Journal of Biosciences·Aarohi DharwadkarAditi Pant
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sarvind Mani Tripathi, Ravishankar Ramachandran

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