Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae

ACS Chemical Biology
Anjaneya S RavipatiDavid J Craik

Abstract

Cyclotides are macrocyclic proteins produced by plants for host defense. Although they occur sparsely in other plant families, cyclotides have been detected in every Violaceae plant species so far screened. Many of the Violaceae species examined until now have been from closely related geographical regions or habitats. To test the hypothesis that cyclotides are ubiquitous in this family, two geographically isolated (and critically endangered) species of Australasian Violaceae, namely Melicytus chathamicus and M. latifolius, were examined. Surprisingly, we discovered a suite of cyclotides possessing novel sequence features, including a lysine-rich nature, distinguishing them from "conventional" cyclotides and suggesting that they might have different physiological activities in plants to those reported to date. The newly discovered cyclotides were found to bind to lipid membranes and were cytotoxic against cancer cell lines but had low toxicity against red blood cells, which is advantageous for potential therapeutic applications. This suite of novel Lys-rich cyclotides emphasizes the broad diversity of cyclotides in Violaceae species.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D SuckauM Przybylski
Aug 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·D J CraikC Waine
Apr 9, 2001·The Journal of Biological Chemistry·M E Felizmenio-QuimioD J Craik
Jan 25, 2002·Nature·Michael Zasloff
Aug 31, 2004·Journal of Natural Products·Ulf GöranssonLars Bohlin
Jan 17, 2007·Toxicon : Official Journal of the International Society on Toxinology·Christian W GruberDavid J Craik
May 31, 2007·Chembiochem : a European Journal of Chemical Biology·Manuel Rey R PlanDavid J Craik
Jan 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Barbara L BarbetaMarilyn A Anderson
Oct 2, 2008·The Plant Cell·Christian W GruberDavid J Craik
May 23, 2009·Organic & Biomolecular Chemistry·Manuela TrabiDavid J Craik
May 28, 2009·Advanced Drug Delivery Reviews·Norelle L DalyDavid J Craik
Jun 6, 2009·The Journal of Biological Chemistry·Yen-Hua HuangDavid J Craik
Jun 22, 2010·Biopolymers·Michelle L ColgraveDavid J Craik
Apr 8, 2011·Neoplasia : an International Journal for Oncology Research·Jason H Stafford, Philip E Thorpe
Aug 30, 2011·The Journal of Biological Chemistry·Laura CascalesDavid J Craik
Jan 21, 2012·ACS Chemical Biology·Sónia Troeira Henriques, David J Craik
Jun 16, 2012·The Journal of Biological Chemistry·Aaron G PothDavid J Craik
Jun 16, 2012·The Journal of Biological Chemistry·Ulf GöranssonK Johan Rosengren
Aug 3, 2012·The Journal of Biological Chemistry·Sónia Troeira HenriquesDavid J Craik
Aug 8, 2014·Chembiochem : a European Journal of Chemical Biology·Sónia Troeira HenriquesDavid J Craik

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Citations

Aug 25, 2016·Biopolymers·Nicolau Brito da CunhaSimoni Campos Dias
Jan 14, 2017·Biochemistry·Sónia Troeira Henriques, David J Craik
May 5, 2017·Journal of Natural Products·Anjaneya S RavipatiDavid J Craik
May 26, 2017·Chembiochem : a European Journal of Chemical Biology·Andrew Gould, Julio A Camarero
Jan 23, 2018·Chemistry : a European Journal·Alexandra M Webster, Steven L Cobb
Dec 23, 2016·Biopolymers·Shaikh Jamal UddinUlf Göransson
Apr 24, 2019·Biomedicines·Julio A Camarero, Maria Jose Campbell
Mar 7, 2020·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lovekesh MehtaSanjay Kumar
May 18, 2020·The Journal of Biological Chemistry·Qingdan DuDavid J Craik
Feb 9, 2020·Antibiotics·Andrey ShelenkovTatyana Odintsova
Nov 7, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Dimitris MatiadisMarina Sagnou
Nov 8, 2017·Bioorganic & Medicinal Chemistry Letters·Julio A Camarero
Apr 2, 2018·International Journal of Biological Macromolecules·Mohammed Al SaiqaliBhima Bhukya
May 22, 2020·Journal of Natural Products·Tien T DangDavid J Craik
Sep 30, 2017·ACS Infectious Diseases·Ingrid A EdwardsMatthew A Cooper

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