Lysine Succinylation and Acetylation in Pseudomonas aeruginosa

Journal of Proteome Research
Charlotte GaviardJulie Hardouin

Abstract

Pseudomonas aeruginosa is a multi-drug-resistant human opportunistic pathogen largely involved in nosocomial infections. Unfortunately, effective antibacterial agents are lacking. Exploring its physiology at the post-translational modifications (PTMs) level may contribute to the renewal of combat tactics. Recently, lysine succinylation was discovered in bacteria and seems to be an interesting PTM. We present the first succinylome and acetylome of P. aeruginosa PA14 cultured in the presence of four different carbon sources using a 2D immunoaffinity approach coupled to nanoliquid chromatography tandem mass spectrometry. A total of 1520 succinylated (612 proteins) and 1102 acetylated (522 proteins) lysine residues were characterized. Citrate was the carbon source in which we identified the higher number of modified proteins. Interestingly, 622 lysine residues (312 proteins) were observed either acetylated or succinylated. Some of these proteins, were involved in virulence, adaptation, resistance, and so on. A label-free quantification points out the existence of different protein forms for a same protein (unmodified, succinylated or acetylated) and suggests different abundance as a function of the carbon sources. This work is a pr...Continue Reading

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Citations

Aug 28, 2018·Expert Review of Proteomics·Charlotte GaviardJulie Hardouin
Sep 6, 2019·Nature Reviews. Microbiology·Boris MacekIvan Mijakovic
Jul 23, 2019·Science Advances·Hanyang DongKai Zhang
Dec 26, 2018·Expert Review of Proteomics·Issa DialloSandrine Bourgoin-Voillard
May 12, 2020·Frontiers in Microbiology·Benjamin RémyÉric Chabrière
Sep 10, 2020·Toxins·Salomé Sauvage, Julie Hardouin
Dec 5, 2020·Chemistry : a European Journal·Samuel OforiSamuel G Awuah
Nov 6, 2020·Science Progress·Suzanne Forrest, Martin Welch
Feb 25, 2021·Nature Communications·Fatemeh AskarianGustav Vaaje-Kolstad
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Fuyuan ZengJichang Jian
Sep 24, 2020·Journal of Proteome Research·Valerie J Carabetta
May 25, 2021·Biochemical and Biophysical Research Communications·Yuxuan MiaoXiangmin Lin
May 22, 2021·Ecotoxicology and Environmental Safety·Zhijuan HuangChaoliang Lei
Aug 11, 2021·Biochemical Society Transactions·Lok ManStuart J Cordwell
Aug 25, 2021·MSystems·Jackson Luu, Valerie J Carabetta

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