PMID: 2123868Dec 1, 1990Paper

Lysine uptake and exchange in Corynebacterium glutamicum

Journal of Bacteriology
S Bröer, Reinhard Krämer

Abstract

Resting cells of Corynebacterium glutamicum (ATCC 13032) accumulate [14C]lysine by a transport system with a relatively high affinity (10 microMs) and a low maximum velocity (0.15 nmol/min per mg [dry weight]). Uptake of lysine was not inhibited by uncouplers or by ionophores affecting the ion gradients and the energetic state of the cell. Analysis of intracellular amino acid concentrations during the transport reaction as well as kinetic studies revealed that the observed uptake of lysine in fact represents a homologous antiport between extracellular [14C]lysine and intracellular unlabeled lysine. Intracellular [14C]lysine could only be released by the addition of unlabeled lysine to the bacterial suspension. In contrast to this homologous antiport reaction, we observed net uptake of lysine in lysine-depleted cells of a lysine auxotrophic strain. This net uptake was found to be electrogenic and could also be observed as a heterologous antiport reaction in wild-type cells under particular conditions. In this case exchange was mediated between internal lysine and external alanine, isoleucine, or valine. This antiport was electrogenic, since the substrates differ in charge. The cells can switch between electroneutral homologous e...Continue Reading

References

Mar 1, 1989·Journal of Bacteriology·A J DriessenW N Konings
Jan 1, 1989·Archives of Microbiology·H EbbighausenR Krämer
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J DriessenW Konings
Sep 1, 1987·Journal of Bacteriology·J Thompson
Jan 1, 1987·Journal of Bacteriology·J Reizer, M H Saier
Jun 1, 1973·Journal of Biochemistry·I ShiioN Kashima
May 15, 1974·European Journal of Biochemistry·D F Niven, W A Hamilton

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Citations

Nov 15, 1991·European Journal of Biochemistry·S Bröer, R Krämer
Dec 1, 1991·Molecular Microbiology·A H Seep-FeldhausA Pühler
Sep 24, 2019·Biotechnology and Bioengineering·Alberte RegueiraMiguel Mauricio-Iglesias
Jan 1, 1994·Antonie van Leeuwenhoek·W N KoningsH W van Veen
Feb 1, 1992·FEMS Microbiology Reviews·W N KoningsA J Driessen
May 15, 1996·Annals of the New York Academy of Sciences·L EggelingH Sahm
Dec 1, 1992·Journal of Bacteriology·J Ruhrmann, R Krämer
Nov 2, 1993·Biochimica Et Biophysica Acta·B Poolman, W N Konings
Nov 23, 1994·Biochimica Et Biophysica Acta·J RuhrmannR Krämer

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