Lysines close to the Rous sarcoma virus late domain critical for budding

Journal of Virology
Jared L SpidelJ W Wills

Abstract

The release of retroviruses from the plasma membrane requires host factors that are believed to be recruited to the site of budding by the late (L) domain of the virus-encoded Gag protein. The L domain of Rous sarcoma virus (RSV) has been shown to interact with a ubiquitin (Ub) ligase, and budding of this virus is dependent on Ub. RSV is similar to other retroviruses in that it contains approximately 100 molecules of Ub, but it is unique in that none of these molecules has been found to be conjugated to Gag. If transient ubiquitination of RSV Gag is required for budding, then replacement of the target lysine(s) with arginine should prevent the addition of Ub and reduce budding. Based on known sites of ubiquitination in other viruses, the important lysines would likely reside near the L domain. In RSV, there are five lysines located just upstream of the L domain in a region of the matrix (MA) protein that is dispensable for membrane binding, and replacement of these with arginine (mutant 1-5KR) reduced budding 80 to 90%. The block to budding was found to be on the plasma membrane; however, the few virions that were released had normal size, morphology, and infectivity. Budding was restored when any one of the residues was change...Continue Reading

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Citations

Feb 28, 2007·Nature Reviews. Microbiology·Stephen P Goff
Nov 10, 2006·Journal of Virology·Jared L SpidelJohn W Wills
Feb 27, 2009·Journal of Virology·Mayra L GarciaMichael D Robek
Jan 20, 2012·Journal of Virology·Megan S HarrisonAnthony P Schmitt
Feb 10, 2012·Journal of Virology·Mariju F BaluyotMarc C Johnson
Jul 5, 2005·Journal of Virology·Eva Gottwein, Hans-Georg Kräusslich
Oct 30, 2009·Advances in Virology·Andrew Pincetic, Jonathan Leis
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maria ZhadinaPaul D Bieniasz
Jul 25, 2007·Traffic·Juan Martin-Serrano
Sep 27, 2008·Traffic·Anjali JoshiEric O Freed
Jul 5, 2011·Traffic·S Brookhart Shields, Robert C Piper
Dec 9, 2003·Journal of Biology·Margaret Q WangStephen P Goff
Sep 17, 2013·Cell Host & Microbe·Jörg Votteler, Wesley I Sundquist
Jun 18, 2017·Biochemical Society Transactions·Edward J Scourfield, Juan Martin-Serrano
Jan 16, 2009·Biochemical Society Transactions·Jeremy G Carlton, Juan Martin-Serrano
May 1, 2020·Journal of Biomolecular Structure & Dynamics·Raied A BadierahElrashdy M Redwan
Apr 20, 2019·International Journal of Molecular Sciences·Liudmila A SeveryanovaAlexey A Kriukov
Apr 4, 2021·International Journal of Molecular Sciences·Charlotte BussienneSerena Bernacchi

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