Lysis delay and burst shrinkage of coliphage T7 by deletion of terminator Tφ reversed by deletion of early genes

Journal of Virology
Huong Minh Nguyen, Changwon Kang

Abstract

Bacteriophage T7 terminator Tϕ is a class I intrinsic terminator coding for an RNA hairpin structure immediately followed by oligo(U), which has been extensively studied in terms of its transcription termination mechanism, but little is known about its physiological or regulatory functions. In this study, using a T7 mutant phage, where a 31-bp segment of Tϕ was deleted from the genome, we discovered that deletion of Tϕ from T7 reduces the phage burst size but delays lysis timing, both of which are disadvantageous for the phage. The burst downsizing could directly result from Tϕ deletion-caused upregulation of gene 17.5, coding for holin, among other Tϕ downstream genes, because infection of gp17.5-overproducing Escherichia coli by wild-type T7 phage showed similar burst downsizing. However, the lysis delay was not associated with cellular levels of holin or lysozyme or with rates of phage adsorption. Instead, when allowed to evolve spontaneously in five independent adaptation experiments, the Tϕ-lacking mutant phage, after 27 or 29 passages, recovered both burst size and lysis time reproducibly by deleting early genes 0.5, 0.6, and 0.7 of class I, among other mutations. Deletion of genes 0.5 to 0.7 from the Tϕ-lacking mutant ph...Continue Reading

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Citations

Oct 1, 2015·Microbiology·Amol Arunrao Pohane, Vikas Jain
Jul 9, 2016·The Journal of Antimicrobial Chemotherapy·Nicolas DufourUNKNOWN ColoColi group
Mar 25, 2015·Critical Care Medicine·Nicolas DufourJean-Damien Ricard
Feb 3, 2021·Antibiotics·Fatma AbdelrahmanAyman El-Shibiny

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