Abstract
In the generation of cytotoxic effector cells specific for influenza A virus-infected lymphocytes, three donors have given an unusual pattern of lytic activity, killing HLA-mismatched target cells. This has been analyzed in detail for one donor and one of the other two shows similar results. Activation only requires culture in medium between 1 and 4 days and parallels development of cell line K562-directed natural killer cells. Target lymphocytes do not need to be virus-infected and appear to be normal lymphocytes. The effector cells carry the surface markers T3 and T8 defined by OKT3/anti-Leu4 and OKT8/anti-Leu2a monoclonal antibodies, respectively. Unlike HLA class 1-restricted or -directed cytotoxic T cells, neither anti-Leu2a/nor anti-Leu4 blocked killing in the absence of complement. MHM23, a monoclonal antibody specific for the human lymphocyte function antigen, blocked lysis. The results indicate that these effector cells are related to cytotoxic T lymphocytes, but can lyse allogeneic target cells through a different recognition process. There is some specificity because autologous cells were not killed.
References
Jan 24, 1979·Medical Microbiology and Immunology·H W KrethG Eckert
Jan 1, 1979·Advances in Immunology·R M Zinkernagel, P C Doherty
Sep 1, 1979·European Journal of Immunology·D Charmot, C Mawas
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Svedmyr, M Jondal
Dec 1, 1979·The Journal of Experimental Medicine·M LipinskiM Fellous
Oct 1, 1977·The Journal of Experimental Medicine·L H PerrinM B Oldstone
May 18, 1978·Nature·K L YapI F McKenzie
Oct 1, 1978·European Journal of Immunology·A J McMichael, B A Askonas
Dec 1, 1978·The Journal of Experimental Medicine·A McMichael
May 1, 1978·The Journal of Experimental Medicine·G Trinchieri, D Santoli
Jan 1, 1979·Immunological Reviews·R B HerbermanP Puccetti
Oct 11, 1974·Nature·R M Zinkernagel, P C Doherty
Feb 1, 1981·The Journal of Experimental Medicine·J A LedbetterL A Herzenberg
Oct 1, 1980·Human Immunology·W E BiddisonS Shaw
Sep 1, 1981·The Journal of Experimental Medicine·N MinatoB R Bloom
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R L EvansR A Good
Nov 27, 1980·Nature·J M Zarling, P C Kung
Jan 1, 1982·European Journal of Immunology·M PierresP Golstein
May 1, 1980·Springer Seminars in Immunopathology·A J McMichael
Oct 15, 1981·International Journal of Cancer. Journal International Du Cancer·G YogeeswaranR M Welsh
Apr 1, 1981·Immunology Today·E L Reinherz, S F Schlossman
Citations
Oct 1, 1996·Journal of Medical Virology·I N MbawuikeR B Couch
Jul 20, 1999·Journal of Immunological Methods·J SoltysM T Quinn
Jan 1, 1993·Viral Immunology·I N MbawuikeR B Couch
Sep 1, 1985·Immunobiology·M T BejaranoE Klein
Feb 17, 2005·Experimental Parasitology·Dylan R Pillai, Kevin C Kain
Dec 1, 1982·European Journal of Immunology·L E WallaceM A Epstein
Mar 1, 1983·European Journal of Immunology·J E HildrethA J McMichael
Nov 24, 1999·Infection and Immunity·J Soltys, M T Quinn
Nov 1, 1986·Infection and Immunity·M CarlG A Dasch