PMID: 6965234Jan 1, 1982Paper

Lysis of autologous human melanoma cells by in vitro allosensitized peripheral blood lymphocytes

Cancer Immunology, Immunotherapy : CII
G FossatiG Parmiani

Abstract

Peripheral blood lymphocytes (PBL) of melanoma patients were sensitized in vitro with lymphocytes of a single donor or with a pool of lymphocytes of 5-20 different donors. After 6-7 days, the cytotoxic activity of the sensitized PBL was tested against cultured autologous tumor cells and lymphocytes in a 51Cr-release assay. Tumor lysis was observed in 13 of 16 cases in which patients' PBL (Pt-PBL) were stimulated by a pool of allogeneic lymphocytes and in five out of seven cases when single sensitization was performed. In no case was lysis of autologous normal lymphocytes or blasts seen. Cultivation of Pt-PBL with irradiated autologous tumor cells never led to the induction of lymphocytes cytotoxic to melanoma cells. Lysability by pool-activated autologous Pt-PBL of fresh cryopreserved tumor cells was compared to that of short-term cultured tumor cells, and no significant differences were observed. Cold-target inhibition experiments indicated that the cytotoxicity of Pt-PBL was tumor-restricted since only autologous melanoma cells but not lymphocytes were able to inhibit the reaction. These results indicate that activation of Pt-PBL is necessary in order to elicit or amplify their antitumor activity.

Citations

Jan 1, 1984·Cancer Immunology, Immunotherapy : CII·B LeshemE Kedar
Nov 1, 1989·Clinical & Experimental Metastasis·L LarizzaG Fossati
Jan 1, 1990·Cancer Genetics and Cytogenetics·G SozziG Della Porta
Jan 1, 1989·International Journal of Cell Cloning·K Pantel, A Nakeff
Oct 15, 1986·International Journal of Cancer. Journal International Du Cancer·A AnichiniG Parmiani
Jan 1, 1986·International Journal of Cell Cloning·P J Quesenberry
May 15, 1985·International Journal of Cancer. Journal International Du Cancer·A AnichiniG Parmiani
Dec 15, 1986·International Journal of Cancer. Journal International Du Cancer·A BalsariG Parmiani
Jun 15, 1987·International Journal of Cancer. Journal International Du Cancer·G FossatiG Parmiani
Apr 15, 1987·International Journal of Cancer. Journal International Du Cancer·T E RobertsM Moore
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May 15, 1984·International Journal of Cancer. Journal International Du Cancer·G FossatiG Parmiani
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·M HérinT Boon
Aug 15, 1988·International Journal of Cancer. Journal International Du Cancer·G FossatiG Parmiani

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