Lysis of surface-localized fibrin clots by adsorbed plasminogen in the presence of tissue plasminogen activator

Biomaterials
K A WoodhouseJ L Brash

Abstract

The ability of plasminogen adsorbed from buffer onto sulphonated silica glass or lysine-derivatized silica glass to lyse fibrin I clots has been investigated. Clots were formed around the test surface by adding reptilase to fibrinogen solutions in which the surfaces were immersed. Tissue plasminogen activator (t-PA) was then added and the extent of clot lysis was determined by measuring the levels of the specific plasmin cleavage product of fibrinogen, B beta 1-42 peptide. The data indicate that in the presence of t-PA, B beta 1-42 generation per mole of bound plasminogen on the lysinized material is approximately two-fold higher than on the sulphonated material. It is concluded that a preformed clot may be lysed by adsorbed plasminogen in the presence of t-PA, and that clot lysis is significantly enhanced when the plasminogen is adsorbed via its lysine binding sites.

Citations

May 6, 2011·Colloids and Surfaces. B, Biointerfaces·Dan LiJohn L Brash
Feb 22, 2011·Macromolecular Bioscience·Lin YuanHong Chen
Aug 20, 2003·Journal of Biomedical Materials Research. Part a·W G McClungJ L Brash
Dec 22, 1999·Journal of Biomedical Materials Research·W G McClungJ L Brash
Sep 21, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoli LiuJohn L Brash
Mar 4, 2021·ACS Applied Materials & Interfaces·Fabian ObstalsCesar Rodriguez-Emmenegger
Jun 3, 2011·Journal of Bioscience and Bioengineering·Chi-Hsien LiuHau-Yaun Hou

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