Lysogenic transfer of group A Streptococcus superantigen gene among Streptococci

The Journal of Infectious Diseases
Ivo VojtekEmmanuelle Charpentier

Abstract

A group A Streptococcus (GAS) isolate, serotype M12, recovered from a patient with streptococcal toxic shock syndrome was analyzed for superantigen-carrying prophages, revealing phi149, which encodes superantigen SSA. Sequence analysis of the att-L proximal region of phi149 showed that the phage had a mosaic nature. Remarkably, we successfully obtained lysogenic conversion of GAS clinical isolates of various M serotypes (M1, M3, M5, M12, M19, M28, and M94), as well as of group C Streptococcus equisimilis (GCSE) clinical isolates, via transfer of a recombinant phage phi149::Km(r). Phage phi149::Km(r) from selected lysogenized GAS and GCSE strains could be transferred back to M12 GAS strains. Our data indicate that horizontal transfer of lysogenic phages among GAS can occur across the M-type barrier; these data also provide further support for the hypothesis that toxigenic conversion can occur via lysogeny between species. Streptococci might employ this mechanism specifically to allow more efficient adaptation to changing host challenges, potentially leading to fitter and more virulent clones.

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Citations

Apr 2, 2011·Nature·Elitza DeltchevaEmmanuelle Charpentier
Sep 29, 2011·Nucleic Acids Research·Justyna E KorczynskaEdward J Taylor
Jul 21, 2010·Antimicrobial Agents and Chemotherapy·Maria Chiara Di LucaLuca A Vitali
Aug 21, 2012·BMC Genomics·Kayo OkumuraTohru Miyoshi-Akiyama
May 2, 2014·World Journal of Microbiology & Biotechnology·Sabah A A Jassim, Richard G Limoges
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dana WillnerMatthew Haynes
Aug 16, 2011·The American Journal of the Medical Sciences·Harmony L TynerLarry M Baddour
May 19, 2009·The Journal of Pediatrics·Tomohiro SuenagaNorishige Yoshikawa
Oct 8, 2008·Immunological Reviews·John D Fraser, Thomas Proft
Oct 4, 2015·Cellular Microbiology·Timothy C BarnettMark J Walker
Mar 19, 2011·Current Opinion in Infectious Diseases·Nicola N LynskeyShiranee Sriskandan
Jul 1, 2012·Emerging Microbes & Infections·Samson Sy Wong, Kwok-Yung Yuen
May 1, 2021·Viruses·Dior BeerensEmmanuelle Charpentier

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