Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon

Scientific Reports
Lucía FernándezAna Rodríguez

Abstract

Staphylococcus aureus is a major opportunistic pathogen that commonly forms biofilms on various biotic and abiotic surfaces. Also, most isolates are known to carry prophages in their genomes. With this in mind, it seems that acquiring a better knowledge of the impact of prophages on the physiology of S. aureus biofilm cells would be useful for developing strategies to eliminate this pathogen. Here, we performed RNA-seq analysis of biofilm cells formed by S. aureus RN450 and two derived strains carrying prophages ϕ11 and ϕ80α. The lysogenic strains displayed increased biofilm formation and production of the carotenoid pigment staphyloxanthin. These phenotypes could be partly explained by the differences in gene expression displayed by prophage-harboring strains, namely an activation of the alternative sigma factor (SigB) regulon and downregulation of genes controlled by the Agr quorum-sensing system, especially the decreased transcription of genes encoding dispersion factors like proteases. Nonetheless, spontaneous lysis of part of the population could also contribute to the increased attached biomass. Interestingly, it appears that the phage CI protein plays a role in orchestrating these phage-host interactions, although more r...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 9, 2000·The Journal of Infectious Diseases·C VuongM Otto
May 20, 2003·Journal of Bacteriology·Edward J FeilNicholas P J Day
Jul 31, 2003·Molecular Microbiology·Sherwood Casjens
Aug 23, 2003·Infection and Immunity·Katherine A SchmidtAmbrose L Cheung
Jun 19, 2004·Journal of Bacteriology·Markus BischoffSteven Projan
Jul 28, 2004·Trends in Microbiology·Jodi A Lindsay, Matthew T G Holden
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Harald BrüssowWolf-Dietrich Hardt
Nov 18, 2004·Journal of Bacteriology·Jeremy S WebbStaffan Kjelleberg
Oct 11, 2005·FEMS Microbiology Letters·Alexandra ReschFriedrich Götz
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Kati SeidlMarkus Bischoff
Jun 15, 2007·Journal of Bacteriology·Julia MakhlinYair Aharonowitz
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Carles UbedaRichard P Novick
Dec 18, 2007·The International Journal of Biochemistry & Cell Biology·Ambrose L CheungSandeep Tamber
Mar 19, 2008·Infection and Immunity·Kati SeidlMarkus Bischoff
Apr 26, 2008·PLoS Pathogens·Blaise R Boles, Alexander R Horswill
Dec 3, 2008·Journal of Bacteriology·Nekane MerinoIñigo Lasa
Jan 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laura SelvaJosé R Penadés
May 20, 2009·BMC Microbiology·Kati SeidlBrigitte Berger-Bächi
Sep 25, 2009·Journal of Virology·Revathi GovindJoe A Fralick
Nov 4, 2009·Microbes and Infection·Miguel MartíJosé R Penadés
Jul 17, 2010·Nature Reviews. Microbiology·Richard P NovickJose R Penadés
Oct 22, 2010·The ISME Journal·Julia GödekeKai M Thormann
Jan 27, 2011·Nature Communications·Xiaoxue WangThomas K Wood
Aug 17, 2011·Molecular Microbiology·Laura CookGary Dunny
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Breck A DuerkopLora V Hooper
Jan 30, 2013·Antimicrobial Agents and Chemotherapy·Victoria J SavageAlex J O'Neill
Apr 20, 2013·Biofouling·Zeinab HosseinidoustTheo G M van de Ven
Apr 25, 2013·Virulence·Louis-Charles Fortier, Ognjen Sekulovic
May 11, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Guoqing Xia, Christiane Wolz
Jun 4, 2013·Applied and Environmental Microbiology·Stuart AinsworthDouwe van Sinderen
Jan 25, 2014·International Journal of Medical Microbiology : IJMM·Dorte FreesHanne Ingmer
Dec 17, 2014·Applied and Environmental Microbiology·Ognjen Sekulovic, Louis-Charles Fortier
Nov 3, 2015·Research in Microbiology·Marcus Lívio Varella CoelhoMaria do Carmo de Freire Bastos
Dec 3, 2015·Environmental Microbiology·Lejla ImamovicMaite Muniesa

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE111012

Methods Mentioned

BETA
RNA-seq
phosphotransferase
RNAseq
electrophoresis
reverse transcription-PCR

Software Mentioned

EDGE
BLASTP
pro
BowTie2

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.