Lysolipids do not inhibit influenza virus fusion by interaction with hemagglutinin.

The Journal of Biological Chemistry
Bolormaa BaljinnyamAndreas Herrmann

Abstract

The interaction of a spin-labeled lysophosphatidylcholine analog with intact and bromelain-treated influenza viruses as well as with the bromelain-solubilized hemagglutinin ectodomain has been studied. The inhibition of fusion of influenza viruses with erythrocytes by the lysophosphatidylcholine analog was similar to that observed for non-labeled lysophosphatidylcholine. Only a weak interaction of the lysophosphatidylcholine analog with the hemagglutinin ectodomain was observed even upon triggering the conformational change of the ectodomain at a low pH. A significant interaction of spin-labeled lysophosphatidylcholine with the hemagglutinin ectodomain of intact viruses was observed neither at neutral nor at low pH, whereas a strong interaction of the lipid analog with the viral lipid bilayer was evident. We suggest that the high number of lipid binding sites of the virus bilayer and their affinity compete efficiently with binding sites of the hemagglutinin ectodomain. We conclude that the inhibition of influenza virus fusion by lysolipids is not mediated by binding to the hemagglutinin ectodomain, preventing its interaction with the target membrane. The results unambiguously argue for an inhibition mechanism based on the actio...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·R BlumenthalM J Clague
Jan 1, 1990·Annual Review of Physiology·J M White
Jan 1, 1989·European Biophysics Journal : EBJ·M M KozlovY A Chizmadzhev
Jan 1, 1987·Annual Review of Biochemistry·D C Wiley, J J Skehel
Oct 5, 1987·Biochimica Et Biophysica Acta·L V ChernomordikY A Chizmadzhev
Dec 8, 1995·The Journal of Biological Chemistry·S Günther-AusbornT Stegmann
Nov 17, 1995·The Journal of Biological Chemistry·J LünebergA Herrmann
Jul 1, 1995·The Journal of Membrane Biology·L ChernomordikJ Zimmerberg
Feb 22, 1994·Biochemistry·P L YeagleT D Flanagan
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·J ZimmerbergL V Chernomordik
Jul 15, 1996·Chemistry and Physics of Lipids·L Chernomordik
May 20, 1999·Molecular Membrane Biology·L V ChernomordikJ Zimmerberg
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN

❮ Previous
Next ❯

Citations

Oct 7, 2003·Annual Review of Biochemistry·Leonid V Chernomordik, Michael M Kozlov
May 29, 2015·Archives of Virology·Shashank TripathiSunil K Lal
Sep 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Cédric GodefroyPierre-Emmanuel Milhiet
Nov 9, 2006·The Journal of Membrane Biology·S OhkiF V Bright

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.