Lysophosphatidic Acid and IL-6 Trans-signaling Interact via YAP/TAZ and STAT3 Signaling Pathways in Human Trabecular Meshwork Cells.

Investigative Ophthalmology & Visual Science
Felix Yemanyi, VijayKrishna Raghunathan

Abstract

Lysophosphatidic acid (LPA) and soluble interleukin-6 receptor (sIL6R) are elevated in primary open angle glaucoma (POAG). LPA and IL6 modulate in response to biomechanical stimuli and converge on similar fibrotic phenotypes. Thus, we determined whether LPA and IL6 trans-signaling (IL6/sIL6R) interact via Yes-associated protein (YAP)/Transcriptional coactivator with a PDZ-binding motif (TAZ) or Signal transducer and activator of transcription 3 (STAT3) pathways in human trabecular meshwork (hTM) cells. Confluent primary hTM cells were serum starved for 24 hours, and treated with vehicle, LPA (20 µM), IL6 (100 ng/mL)/sIL6R (200 ng/mL), or both (LPA + IL6/sIL6R) for 24 hours, with or without a YAP inhibitor (verteporfin; 2 µM) or STAT3 inhibitor (2 µM). Expression of key receptors and ligands, signaling mediators, actomyosin machinery, cell contractility, and extracellular matrix (ECM) targets of both signaling pathways was determined by immunocytochemistry, RT-qPCR, and Western blotting. LPA and IL6 trans-signaling coupling overexpressed/activated receptors and ligands, glycoprotein-130, IL6, and autotaxin; signaling mediators, YAP, TAZ, Pan-TEAD, and phosphorylated STAT3 (pSTAT3); actomyosin and contractile machinery components...Continue Reading

References

Aug 1, 1991·Acta Ophthalmologica·A G KonstasW R Lee
Feb 1, 1989·Mechanisms of Ageing and Development·M A Babizhayev, M W Brodskaya
Jan 1, 1989·Experimental Eye Research·E Lütjen-DrecollJ Mollenhauer
May 1, 1994·Experimental Eye Research·R C TripathiB J Tripathi
May 1, 1996·The British Journal of Ophthalmology·H A Quigley
Oct 9, 2002·Archives of Ophthalmology·Anders HeijlUNKNOWN Early Manifest Glaucoma Trial Group
Mar 17, 2004·The Journal of Biological Chemistry·Perumal SivashanmugamYehia Daaka
Jun 30, 2004·Investigative Ophthalmology & Visual Science·Priyatham Sai MettuP Vasantha Rao
Feb 12, 2005·The Journal of Biological Chemistry·Marcella DebiddaYi Zheng
Aug 16, 2005·Science·Jeong-Ho HongMichael B Yaffe
Oct 19, 2005·Biochemical and Biophysical Research Communications·Paloma B LitonPedro Gonzalez
Apr 27, 2007·Progress in Lipid Research·Laurens A van Meeteren, Wouter H Moolenaar
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
Oct 28, 2008·Investigative Ophthalmology & Visual Science·Mary-Jo HoareAbbot F Clark
Dec 31, 2008·Experimental Eye Research·Ozan-Yüksel Tektas, Elke Lütjen-Drecoll
Nov 27, 2009·American Journal of Physiology. Cell Physiology·Padmanabhan P Pattabiraman, Ponugoti Vasantha Rao
Jan 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Feng HaoMei-Zhen Cui
Jan 12, 2011·Investigative Ophthalmology & Visual Science·Julie A LastPaul Russell
Feb 19, 2011·Investigative Ophthalmology & Visual Science·John G BrowneJohn K Crean
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Dec 14, 2011·Investigative Ophthalmology & Visual Science·Yasuyuki TakaiAkihiro Ohira
Jan 21, 2012·Current Opinion in Ophthalmology·W Daniel Stamer, Ted S Acott
May 1, 2012·The American Journal of Pathology·Benjamin JunglasRudolf Fuchshofer
Dec 22, 2012·Investigative Ophthalmology & Visual Science·Vijay Krishna RaghunathanPaul Russell
Sep 14, 2013·Investigative Ophthalmology & Visual Science·Wanda E Medina-OrtizAbbot F Clark
Nov 22, 2014·Nature Reviews. Molecular Cell Biology·Caroline BonnansZena Werb
Dec 17, 2014·Journal of Biochemistry·Shizu AikawaJunken Aoki
Mar 10, 2015·Current Opinion in Immunology·Christoph GarbersStefan Rose-John
Apr 9, 2015·Development·Xiaoyan ShengJerold Chun
May 10, 2015·Experimental Eye Research·Padmanabhan P PattabiramanP Vasantha Rao
Aug 20, 2015·The British Journal of Ophthalmology·Venediktos V KapetanakisAlicja R Rudnicka
Sep 12, 2015·Biochemical and Biophysical Research Communications·Wei-Sheng ChenNoorjahan Panjwani
Jan 9, 2016·Cancer Research·Gavin D BrooksBrendan J Jenkins
Apr 20, 2016·Experimental Eye Research·Hannah C WebberWeiming Mao
May 12, 2016·Investigative Ophthalmology & Visual Science·Priya GuptaDavid S Friedman
Jul 9, 2016·Arthritis & Rheumatology·Flavia V CastelinoAndrew M Tager
Jul 23, 2016·Experimental Eye Research·W Daniel Stamer, Abbot F Clark

❮ Previous
Next ❯

Citations

May 20, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Amy O'ReganSarah B Eivers

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.