Lysophosphatidic acid induces early growth response-1 (Egr-1) protein expression via protein kinase Cδ-regulated extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) activation in vascular smooth muscle cells.

The Journal of Biological Chemistry
Takuya IyodaMei-Zhen Cui

Abstract

Lysophosphatidic acid (LPA) modulates vascular cell function in vitro and in vivo via regulating the expression of specific genes. Previously, we reported that a transcriptional mechanism controls LPA-induced expression of Egr-1 in vascular smooth muscle cells. Egr-1 is a master transcription factor mediating the expression of various genes that have been implied to modulate a broad spectrum of vascular pathologies. In this study, we determined the essential intracellular signaling pathway leading to LPA-induced Egr-1 expression. Our data demonstrate that activation of ERK1/2 and JNK, but not p38 MAPK, is required for LPA-induced Egr-1 expression in smooth muscle cells. We provide the first evidence that MEK-mediated JNK activation leads to LPA-induced gene expression. JNK2 is required for Egr-1 induction. Examining the upstream kinases that mediate ERK and JNK activation, leading to Egr-1 expression, we found that LPA-induced activation of MAPKs and expression of Egr-1 are dependent on PKC activation. We observed that LPA rapidly activates PKCδ and PKCθ. Overexpression of dominant-negative PKCδ, but not dominant-negative PKCθ, diminished activation of ERK and JNK and blocked LPA-induced expression of Egr-1 mRNA and protein. We...Continue Reading

References

Feb 28, 1994·Biochemical and Biophysical Research Communications·M GschwendtF Marks
May 1, 1993·The Biochemical Journal·T EichholtzW H Moolenaar
May 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·R CazzolliC Schmitz-Peiffer
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Mei-Zhen CuiXuemin Xu
Jun 24, 2004·Reviews in Endocrine & Metabolic Disorders·Florian BlaschkeRonald E Law
Feb 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jan RuppMatthias Maass
Feb 4, 2006·Circulation Research·Levon M Khachigian
Dec 5, 2006·Biochemical Pharmacology·Cécile SpringaelOlivier Vosters
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wen-Cheng ChungJa Seok Koo
Jan 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Feng HaoMei-Zhen Cui
Dec 14, 2011·Clinical Lipidology·Mei-Zhen Cui

❮ Previous
Next ❯

Citations

Dec 4, 2012·International Journal of Molecular Sciences·Judith-Irina Pagel, Elisabeth Deindl
Jul 15, 2015·Algorithms for Molecular Biology : AMB·Pegah KhosraviGary D Bader
Feb 19, 2014·Journal of Molecular and Cellular Cardiology·Tomomi E KimuraMark Bond
Oct 6, 2012·Biochimica Et Biophysica Acta·Jing ShiXuemin Xu
May 2, 2017·Canadian Journal of Physiology and Pharmacology·Estelle R Simo-CheyouAshok K Srivastava
Dec 7, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Caroline ArnoldThomas Korff
Apr 27, 2018·Cell Biology International·Xiaofan LiShufeng Li
Mar 12, 2016·Nature Communications·Christoph WeigelOdilia Popanda
Jul 7, 2018·Experimental and Therapeutic Medicine·Shi WangLiting Chen
Apr 22, 2016·American Journal of Physiology. Cell Physiology·Justin R SysolRoberto F Machado
Feb 28, 2020·Biochemistry Research International·Wanqiong Yuan, Chunli Song
Oct 30, 2020·Journal of Neurochemistry·Kazue Hisaoka-NakashimaNorimitsu Morioka
Mar 8, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paola SecchieroGiorgio Zauli

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.