PMID: 9422348Jan 9, 1998Paper

Lysophosphatidic acid induces necrosis and apoptosis in hippocampal neurons

Journal of Neurochemistry
F W HoltsbergS M Steiner

Abstract

A diverse body of evidence indicates a role for the lipid biomediator lysophosphatidic acid (LPA) in the CNS. This study identifies and characterizes the induction of neuronal death by LPA. Treatment of cultured hippocampal neurons from embryonic rat brains with 50 microM LPA resulted in neuronal necrosis, as determined morphologically and by the release of lactate dehydrogenase. A concentration of LPA as low as 10 microM led to the release of lactate dehydrogenase. In contrast, treatment of neurons with 0.1 or 1.0 microM LPA resulted in apoptosis, as determined by chromatin condensation. In addition, neuronal death induced by 1 microM LPA was characterized as apoptotic on the basis of terminal dUTP nick end-labeling (TUNEL) staining, externalization of phosphatidylserine, and protection against chromatin condensation, TUNEL staining, and phosphatidylserine externalization by treatment with N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone, a broad-spectrum inhibitor of caspases, i.e., members of the interleukin-1beta converting enzyme family. Studies with antagonists of ionotropic glutamate receptors did not indicate a significant role for these receptors in apoptosis induced by 1 microM LPA. LPA (1 microM) also induced a de...Continue Reading

Citations

Feb 16, 1999·Brain Pathology·D L TaylorH Mehmet
Nov 1, 2001·Cell Death and Differentiation·F PhilippoussisP Hugo
Jul 27, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nicolas BlondeauCatherine Heurteaux
Aug 2, 2003·Nature Reviews. Cancer·Gordon B Mills, Wouter H Moolenaar
Dec 17, 2004·Journal of Cellular and Molecular Medicine·Yoshihiro Higuchi
Dec 7, 2002·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Nobuyuki FukushimaJerold Chun
May 20, 2000·Annals of the New York Academy of Sciences·M S BeerG Mcallister
Jun 21, 2005·Annals of the New York Academy of Sciences·Angela R Kamps, Clark R Coffman
Jul 10, 2009·American Journal of Physiology. Cell Physiology·Helmuth A SánchezJuan C Sáez
Mar 9, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Olga SuckauAnja U Bräuer
Jan 5, 2000·Journal of Neurochemistry·P S Sastry, K S Rao
Jul 20, 2004·Journal of Cellular Biochemistry·Nobuyuki Fukushima
Nov 24, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sonia BraultSylvain Chemtob
Dec 31, 2008·Glia·Abdullah Md SheikhJunichi Masuda
May 25, 2010·Cell and Tissue Research·Yona GoldshmitAnn M Turnley
Mar 26, 2016·Journal of Neuroinflammation·Avinash ParimisettyChristian Lefebvre d'Hellencourt
Jan 29, 2000·The European Journal of Neuroscience·P MarinJ Prémont
Jul 20, 2004·Journal of Cellular Biochemistry·Julie D Saba
May 20, 2000·Annals of the New York Academy of Sciences·M R SteinerS M Steiner
Jan 2, 2021·Frontiers in Neurology·Peethambaran ArunJoseph B Long
Jan 31, 2004·The European Journal of Neuroscience·Nicolai E SavaskanRobert Nitsch
Jan 22, 2005·Journal of Neurochemistry·Jae-Kwang JinYong-Sun Kim
Jan 29, 2021·Neuropathology and Applied Neurobiology·Ángela Gento-CaroBernardo Moreno-López
Sep 30, 1998·Journal of Neuroscience Research·F W HoltsbergS M Steiner
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·J R EricksonG B Mills
Aug 26, 1998·Biochimica Et Biophysica Acta·S A SusinG Kroemer
May 20, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Amy O'ReganSarah B Eivers
Jan 18, 2003·Biochimica Et Biophysica Acta·Xiaoqin YeJerold Chun
Oct 14, 2003·Biochemical Pharmacology·Yoshihiro Higuchi
Oct 4, 2005·Cellular Signalling·Shin-Young ParkShalom Avraham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis