Lysophosphatidic acid stimulates phospholipase D activity and cell proliferation in PC-3 human prostate cancer cells

Journal of Cellular Physiology
C QiKathryn E Meier

Abstract

Phospholipase D (PLD) is activated in mammalian cells in response to a variety of growth factors and may play a role in cell proliferation. Lysophosphatidic acid (LPA) is a bioactive metabolite potentially generated as a result of PLD activation. Two human prostate cancer cell lines, PC-3 and LNCaP, express membrane PLD activity. The effects of LPA on PLD activity and proliferation were examined in PC-3 cells, which express hPLD1a/1b. Phorbol 12-myristate 13-acetate (PMA) induced a prolonged activation of PLD, as detected in both intact cells and membranes. LPA induced a transient activation of PLD that was maximal by 10 minutes. The EC50 for LPA-induced PLD activation was approximately 1 microM. Pertussis toxin did not inhibit activation of PLD by LPA or PMA. Ro-31-8220 and bisindolylmaleimide I, inhibitors of protein kinase C, blocked activation by PLD by both PMA and LPA. PMA-induced activation of PLD did not appear to require translocation of PLDs from cytosol to membrane. LPA stimulated proliferation of PC-3 cells with an EC50 of approximately 0.2 microM; this response was not inhibited by pertussis toxin. Perillyl alcohol, an anti-cancer drug, reversibly inhibited proliferation in response to either serum or LPA but did n...Continue Reading

References

Jan 1, 1978·Advances in Lipid Research·M Heller
Jan 1, 1991·Methods in Enzymology·R L Wykle, J C Strum
Apr 1, 1991·Biochemical Society Transactions·M Liscovitch
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S B BocckinoJ H Exton
Jul 21, 1995·The Journal of Biological Chemistry·B E HawesR J Lefkowitz
Jun 2, 1995·The Journal of Biological Chemistry·W H Moolenaar
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·J D Haag, M N Gould
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·D MassenburgM Vaughan
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·G TigyiR Miledi
Jun 15, 1993·Biochemical and Biophysical Research Communications·F ImamuraH Akedo
Apr 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S H Zeisel
Jan 19, 1996·The Journal of Biological Chemistry·S M KnoeppK E Meier
Jan 1, 1996·International Journal of Urology : Official Journal of the Japanese Urological Association·G KimuraY Fujita-Yamaguchi
May 1, 1996·Trends in Pharmacological Sciences·A J MorrisM A Frohman
Apr 1, 1997·Current Opinion in Cell Biology·W H MoolenaarG C Zondag

❮ Previous
Next ❯

Citations

Sep 30, 1998·Journal of Neuroscience Research·F W HoltsbergS M Steiner
Nov 24, 1999·Journal of Cellular Physiology·T C Gibbs, K E Meier
Mar 13, 2001·Biochemical and Biophysical Research Communications·J H HongS K Park
Sep 22, 2007·Cancer Chemotherapy and Pharmacology·Howard H BaileyJames A Stewart
May 19, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Reinhard MüllerNovica M Milićević
Sep 24, 1999·The Journal of Urology·D T PriceL M Luttrell
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·G Tigyi
Feb 5, 2002·Cancer Letters·Sumathi RamachandranSampath Parthasarathy
Mar 26, 2003·Free Radical Biology & Medicine·Sumathi RamachandranSampath Parthasarathy
Jun 19, 2002·Biochimica Et Biophysica Acta·Yuhuan XieKathryn E Meier
Jan 22, 2000·The Biochemical Journal·M LiscovitchX Tang
Sep 19, 2012·Biochimica Et Biophysica Acta·Sunny Yang XiangJoan Heller Brown
Jan 4, 2012·Progress in Lipid Research·Jin-Hyeok JangSung Ho Ryu
Aug 26, 2010·British Journal of Pharmacology·Gabor Tigyi
Feb 18, 2012·Reproduction in Domestic Animals = Zuchthygiene·M S Pérez AguirreburualdeM Córdoba
Jul 24, 2004·Seminars in Cell & Developmental Biology·Saubhik SenguptaYan Xu
Aug 28, 2012·Clinical Biochemistry·Hussain Mohamad AwwadRima Obeid
Mar 22, 2002·Biology of Reproduction·Lygia T Budnik, Amal K Mukhopadhyay
Nov 8, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gizem E GencSaadet Gumuslu
Jun 27, 2002·The Journal of Biological Chemistry·Yuhuan XieKathryn E Meier
Feb 18, 2017·Nature Reviews. Drug Discovery·H Alex BrownCraig W Lindsley
May 20, 2000·Annals of the New York Academy of Sciences·T C GibbsK E Meier
Jul 30, 1999·Biochimica Et Biophysica Acta·K E MeierK M Ella
Aug 28, 2021·Cells·Pravita BalijepalliKathryn E Meier
Nov 11, 2008·Cellular Signalling·Yutong Zhao, Viswanathan Natarajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.