Lysophosphatidylcholine acyltransferase 1 upregulation and concomitant phospholipid alterations in clear cell renal cell carcinoma

Journal of Experimental & Clinical Cancer Research : CR
Yiqing DuTao Xu

Abstract

The involvement of lipid metabolism in tumourigenesis and the progression of clear cell renal cell carcinoma (ccRCC) have been reported. However, the role of phospholipid profile alterations in ccRCC has not yet been systematically explored. In the present study, we compared the phospholipid compositions between ccRCC and paired normal renal tissues. The phospholipid compositions of paired ccRCC and normal renal tissues were evaluated using liquid chromatography tandem mass spectrometry (LC/MS/MS). To evaluate the mRNA and protein levels of lysophosphatidylcholine acyltransferase (LPCAT), which converts lysophosphatidylcholine (LPC) to phosphatidylcholine (PC), qRT-PCR, western blotting and immunohistochemistry were performed. The correlations of LPCAT1 expression with clinicopathological features and prognosis were assessed. In addition, siRNAs were used to knockdown LPCAT1 expression in ccRCC cell lines, and its effect on cell proliferation, cell cycle, migration and invasion were investigated. The phospholipid compositions of ccRCC and normal renal tissues were significantly different. Multiple LPC species were decreased and corresponding PC species were increased in cancer tissues. The mRNA and protein levels of LPCAT1 were...Continue Reading

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Citations

Apr 13, 2018·Molecular Nutrition & Food Research·Li ChenHaidong Zhu
Nov 1, 2018·Annual Review of Physiology·Bo Wang, Peter Tontonoz
Dec 6, 2019·Journal of Clinical Laboratory Analysis·Zheng CaoXiaowei Liu
Feb 23, 2019·Journal of Experimental & Clinical Cancer Research : CR·Chunhua WeiXiaorong Dong
May 18, 2020·Breast Cancer Research and Treatment·Catarina L SilvaMarijana Petković
Oct 12, 2020·Archives of Biochemistry and Biophysics·Yuan-Yuan ChenYing-Yong Zhao
Jan 9, 2021·Cancer & Metabolism·Shilpa R NagarajanAndrew J Hoy
Jul 28, 2020·Advanced Drug Delivery Reviews·Lisa M ButlerJohannes V Swinnen
Apr 23, 2021·Molecular & Cellular Proteomics : MCP·Zhiao ShiBing Zhang
Aug 7, 2021·The Journal of Investigative Dermatology·Yingjian HuangYan Zheng
Oct 1, 2020·ACS Applied Materials & Interfaces·Yali JunQi-Long Wang
Aug 23, 2021·Cancer Cell International·Rong-Quan HeGang Chen
Jul 20, 2021·American Journal of Respiratory and Critical Care Medicine·Peter McErleanAdam J Byrne

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Methods Mentioned

BETA
PCR
Protein Assay
electrophoresis
transfection
PCA
flow cytometry
deacylation

Software Mentioned

Quantity One
MetaboAnalyst
SPSS
ModFit

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