PMID: 11913468Mar 27, 2002Paper

Lysophosphatidylcholine-induced taurine release in HeLa cells involves protein kinase activity

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
I H Lambert, B Falktoft

Abstract

It has recently been demonstrated that exogenous addition of low concentrations (< 15 microM) of lysophosphatidyl choline (LPC, palmitic acid in the sn-1 position) induces a transient increase in taurine efflux from HeLa cells in a process that seems to involve generation of reactive oxygen species (ROS) and tyrosine phosphorylation (J. Membrane Biol. 176 (2000) 175-185). We now demonstrate that LPC also induces release of taurine under isotonic conditions in mouse fibroblast (NIH/3T3) and Ehrlich ascites tumor cells. Furthermore, we show that in the case of HeLa cells addition of the calmodulin antagonist W-7 (50 microM) or the calmodulin-dependent kinase II (CaMKII) inhibitor KN-62 (10 microM) reduces the LPC-induced taurine release under isotonic conditions. Conversely, addition of a standard protein kinase C (PKC) inhibitor chelerythrine (10 microM) leads to a potentiation of the LPC-induced taurine efflux, whereas direct activation of PKC by the phorbol ester PMA has no effect. It is suggested that the putative generation of ROS following addition of LPC is modulated by calmodulin/CaMKII, and that the effect of chelerythrine is more likely related to the ROS production than to PKC inhibition.

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Citations

Aug 22, 2014·Acta Physiologica·I H LambertO H Mortensen
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ian Henry Lambert, Daniel Bloch Hansen
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Jan 10, 2003·American Journal of Physiology. Cell Physiology·Niels ØrtenbladIan Henry Lambert
Oct 16, 2001·Journal of Agricultural and Food Chemistry·I H LambertN Ørtenblad

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