Lysophosphatidylethanolamine in Grifola frondosa as a neurotrophic activator via activation of MAPK.

Journal of Lipid Research
Atsuyoshi NishinaShoei Furukawa

Abstract

We found that Grifola frondosa extracts induced the activation of mitogen-activated protein kinase (MAPK) in cultured PC12 cells, a line of rat pheochromocytoma cells. The active substance was isolated by a few chromatographic steps, including high-performance liquid chromatography, and was identified to be lysophosphatidylethanolamine (LPE) from various structural analyses. LPE from G. frondosa (GLPE) was confirmed to induce the activation of MAPK of cultured PC12 cells and was found to suppress cell condensation and DNA ladder generation evoked by serum deprivation, suggesting that the GLPE had antiapoptotic effects. Moreover, GLPE caused morphological changes in and upregulation of neurofilament M expression of PC12 cells, demonstrating that the GLPE could induce neuronal differentiation of these cells. The activation of MAPK by GLPE was suppressed by AG1478, an antagonist of epidermal growth factor receptor (EGFR), and by U0126, an inhibitor of MAPK kinase (MEK1/2), but not by K252a, an inhibitor of TrkA, or by pertussis toxin. These results demonstrate that GLPE induced the MAPK cascade [EGFR-MEK1/2-extracellular signal-regulated protein kinases (ERK1/2)] of PC12 cells, the activation of which induced neuronal differentiat...Continue Reading

References

Jan 30, 1987·Journal of Chromatography·A A EntezamiK E Daugherty
Feb 1, 1997·Chemosphere·Y NakamuraH Pan-Hou
Mar 4, 2000·Bioorganic & Medicinal Chemistry Letters·K LackeyE R Wood
Jul 10, 2001·The Journal of Biological Chemistry·H P ReuschK Moelling
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Yu KoyamaMamoru Isemura
Sep 5, 2002·Journal of Neuroscience Research·Hisanori ItoShoei Furukawa
Dec 28, 2002·Journal of Natural Products·In-Kyoung LeeIck-Dong Yoo
Jan 18, 2003·Biochimica Et Biophysica Acta·Xiaoqin YeJerold Chun
Feb 15, 2003·The Journal of Biological Chemistry·Phoebe Lin, Richard D Ye
May 24, 2003·Biochemical and Biophysical Research Communications·Chang-Wook LeeSung-Oh Huh
Jul 25, 2003·Trends in Biochemical Sciences·Celine LuquainAndrew J Morris
Dec 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Caius G RaduOwen N Witte
Dec 30, 2003·Journal of Natural Products·Sachiko TsukamotoTomihisa Ohta
Jan 1, 2004·Progress in Brain Research·Francesco AngelucciLuigi Aloe
Mar 6, 2004·Neuromolecular Medicine·Ronald S Duman
Mar 20, 2004·Journal of Agricultural and Food Chemistry·Ho Jin HeoDong-Hoon Shin
May 25, 2004·The Journal of Biological Chemistry·Takehiko YamazakiShigeki Tsuchida
Aug 4, 2004·The Journal of Cell Biology·Rachelle E TomanSarah Spiegel
Dec 14, 2004·The Journal of Biological Chemistry·Fusheng YangGregory M Cole

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Citations

Nov 18, 2009·Chemical Research in Toxicology·Kelly M Wynalda, Robert C Murphy
Dec 29, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Lee-Fang EikVikineswary Sabaratnam
Oct 31, 2015·Critical Reviews in Biotechnology·Mohammad Azizur RahmanNorhaniza Aminudin
Nov 3, 2015·Biological & Pharmaceutical Bulletin·Atsuyoshi NishinaHirokazu Kimura
Aug 24, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Clark J NelsonHannah V Carey
Jul 11, 2013·Cellular Signalling·Soo-Jin ParkDong-Soon Im
Jul 16, 2009·The Biochemical Journal·Shihui GuoMingdong Huang
Apr 19, 2015·Biochemical and Biophysical Research Communications·Jung-Min LeeDong-Soon Im
Oct 24, 2012·Biochimie·Anna Grzelczyk, Edyta Gendaszewska-Darmach
Jun 9, 2016·Mass Spectrometry Reviews·Raju BanduKwang Pyo Kim
Jul 5, 2013·BMC Complementary and Alternative Medicine·Syntyche Ling-Sing SeowVikineswary Sabaratnam
Jun 16, 2016·Biomolecules & Therapeutics·Jung-Min LeeDong-Soon Im
Apr 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Atsuyoshi NishinaHirokazu Kimura
Jan 19, 2017·Journal of Medicinal Food·Chia-Wei PhanVikineswary Sabaratnam
Jun 4, 2014·Journal of Lipid Research·Kumiko MakideJunken Aoki
Nov 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Atsuyoshi NishinaMakoto Makishima
Nov 30, 2018·Journal of Lipid Research·James A WepyLawrence J Marnett
Apr 7, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shirou TabeTatsuji Nishihara
Jul 17, 2010·Expert Reviews in Molecular Medicine·Marc E SeamanKimberly A Kelly
Jul 31, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·J B GreerD Schlenk
Apr 23, 2014·Biomolecules & Therapeutics·Soo-Jin ParkDong-Soon Im
Mar 23, 2017·Nature·Zuzana KeckesovaRobert A Weinberg
Nov 29, 2016·Frontiers in Cellular Neuroscience·Javier G Villamil-OrtizGloria P Cardona-Gómez
Jan 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yasuyuki TamaiYoshiyuki Takei
Oct 18, 2020·Progress in Lipid Research·Shu Ting TanLong N Nguyen
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Youli WangJianmin Yuan

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