Lysophosphatidylserine induces calcium signaling through Ki16425/VPC32183-sensitive GPCR in bone marrow-derived mast cells and in C6 glioma and colon cancer cells

Archives of Pharmacal Research
Kyeok KimDong-Soon Im

Abstract

Lysophosphatidylserine (LPS) can be generated following phosphatidylserine-specific phospholipase A2 activation. The effects of LPS on cellular activities and the identities of its target molecules, however, have not been fully elucidated. In this study, we observed that LPS stimulated intracellular calcium increased in mouse bone marrow-derived mast cells (BMMC), and rat C6 glioma and human HCT116 colon cancer cells and compared the LPS-induced Ca2+ increases with the response by lysophosphatidic acid (LPA), a structurally related bioactive lysolipid. In order to test involvement of signaling molecules in the LPS-induced Ca2+ signaling, we used pertussis toxin (PTX), U73122, and 2-APB, which are specific inhibitors for G proteins, phospholipase C (PLC), and IP3 receptors, respectively. The increases due to LPS and LPA were inhibited by PTX, U-73122 and 2-APB, suggesting that both lipids stimulate calcium signaling via G proteins (Gi/o types), PLC activation, and subsequent IP3 production, although the sensitivity to pharmacological inhibitors varied from complete inhibition to partial inhibition depending on cell type and lysolipid. Furthermore, we observed that Ki16425 completely inhibited an LPS-induced Ca2+ response in thre...Continue Reading

References

Aug 1, 1995·The Biochemical Journal·Y XuG B Mills
Jun 1, 1995·Journal of Cellular Physiology·Y XuG B Mills
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·G TigyiR Miledi
May 12, 1998·The American Journal of Physiology·K LiliomM A Watsky
Jul 8, 1998·Neuroscience Letters·S Lourenssen, M G Blennerhassett
Dec 22, 1999·Trends in Pharmacological Sciences·K R Lynch, D S Im
Jul 13, 2000·Biochemical and Biophysical Research Communications·L R FitzgeraldP Tsui
Dec 1, 2001·Science·T HlaM J Kluk
May 31, 2002·Pharmacological Reviews·Jerold ChunGabor Tigyi
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keiko KawamotoHiroshi Matsuda
Aug 17, 2002·Current Opinion in Immunology·Volker Brinkmann, Kevin R Lynch
Dec 25, 2002·Trends in Pharmacological Sciences·Dong Soon Im
Sep 23, 2003·Molecular Pharmacology·Hideo OhtaFumikazu Okajima
Dec 6, 2003·Journal of Lipid Research·Dong-Soon Im
Jan 28, 2004·Journal of Pharmacological Sciences·Mi-Ran YunDong-Soon Im
Jun 24, 2004·Molecular Pharmacology·Graeme Milligan
Feb 8, 2006·Biochemical and Biophysical Research Communications·Tsukasa SugoMasaaki Mori
May 3, 2006·The Journal of Pharmacology and Experimental Therapeutics·Knut KotarskyBjörn Olde
Jun 16, 2006·The Journal of Biological Chemistry·Chang-Wook LeeJerold Chun
Oct 7, 2006·Biochimica Et Biophysica Acta·Alexander ZaslavskyYan Xu
Dec 19, 2006·The Journal of Biological Chemistry·Keisuke YanagidaTakao Shimizu

❮ Previous
Next ❯

Citations

Aug 12, 2009·The Journal of Biological Chemistry·Laura SturlaElena Zocchi
Oct 24, 2012·Biochimie·Anna Grzelczyk, Edyta Gendaszewska-Darmach
Mar 13, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Astra I ChangAdolfo J de Bold

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

The Biochemical Journal
Lars RitscherTorsten Schöneberg
Biochemical and Biophysical Research Communications
Tsukasa SugoMasaaki Mori
Biochemical and Biophysical Research Communications
Kyoung Sun ParkYoe-Sik Bae
© 2022 Meta ULC. All rights reserved