Lysophospholipids elevate [Ca2+]i and trigger exocytosis in bovine chromaffin cells

Neuropharmacology
Chien-Yuan PanChia-Lin Chen

Abstract

Sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA) are responsible for many physiological functions, including angiogenesis, neuronal survival, and immunity. However, little is known about their effects in modulating the stimulus-secretion coupling in bovine chromaffin cells. The result of PCR showed that at least two receptors (S1P(3) and LPA(1)) were expressed in bovine chromaffin cells. The elevation of [Ca(2+)](i) by S1P was fast and sustaining; but the elevation by LPA was slow and transient. The EC(50) for S1P and LPA in elevating the [Ca(2+)](i) were 0.55+/-0.01 and 0.54+/-0.40microM, respectively. This elevation could be totally blocked by thapsigargin, 2-APB, and U73122. Pertussis toxin pretreatment inhibited about half of the elevation in [Ca(2+)](i) suggesting the involvement of G(i) and other G-proteins. Repetitive [Ca(2+)](i) elevations elicited by S1P, but not LPA, were inhibited by ryanodine. S1P was more effective than LPA in triggering exocytosis as measured by the changes in membrane capacitance. The whole-cell Ca(2+) current was inhibited by both lysophospholipids but Na(+) current was inhibited by S1P only. These results suggest the differential effects of LPA and S1P in releasing Ca(2+) from the ...Continue Reading

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Citations

Oct 26, 2007·Journal of Biomedical Science·Ming-Ling ChenChien-Yuan Pan
Jul 17, 2007·Journal of Neurochemistry·Chien-Yuan PanYit-Tsong Chen
Nov 1, 2011·Biosensors & Bioelectronics·Ko-Shing ChangYit-Tsong Chen
Oct 4, 2016·Biochemical and Biophysical Research Communications·Christopher T TurnerDoug A Brooks
Nov 17, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Seung-Yeol NaDong-Yoon Lim

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