Lysosomal acid phosphatase: activity and isoenzymes in separated normal human blood cells

Clinica Chimica Acta; International Journal of Clinical Chemistry
H J RadzunH Stein

Abstract

The present study was devised to investigate the activity and isoenzymes of lysosomal acid phosphatase in individual normal human blood cells, including the T- and B-population of lymphocytes, with the aim to contribute to the classification of haematopoietic neoplasias on the basis of cell specific isoenzyme patterns. Platelets, erythrocytes, granulocytes, monocytes and T-lymphocytes were isolated from blood by gradient centrifugation or immune adsorption. B-lymphocytes were obtained from human tonsils. After purity control and isolation of lysosomes the concentration of acid phosphatase was assayed using the conventional spectrophotometric method. Isoenzymes were separated by isoelectric focusing on polyacrylamide thin layer slabs. Monocytes revealed the highest activity with 14 mU/10(7) cells, about three times more than granulocytes. T-lymphocytes showed an activity of 2.85 mU/10(7) cells and B-lymphocytes of 1.83 mU/10(7) CELLS. The lowest activity was found in platelets with 0.08 mU/10(7) cells. Granulocytes showed 12 isoenzyme bands, whilst the number for monocyte, B-lymphocytes, T-lymphocytes and platelets were respectively 11, 12, 1 and 4 isoenzyme bands. Thus it became evident that the different blood cell populations...Continue Reading

References

Jan 1, 1978·Acta Haematologica·G A PangalisE Beutler
Jan 1, 1978·Oncology·T M ChuG P Murphy
Apr 1, 1976·British Journal of Haematology·R D Barr, S Perry
Dec 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiK W Lam
Jan 26, 1972·Biochimica Et Biophysica Acta·O Vesterberg
Jan 20, 1973·Lancet·J WybranH H Fudenberg
Apr 1, 1974·European Journal of Immunology·G Brown, M F Greaves
Jan 1, 1966·The Journal of Experimental Medicine·W E Bennett, Z A Cohn
Dec 1, 1968·The Journal of Pharmacy and Pharmacology·R Best, P S Spencer
May 1, 1974·The American Journal of Medicine·L T Yam
Jul 1, 1974·Journal of Immunological Methods·M E Kaplan, C Clark
Jul 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiK W Lam
May 9, 1972·Biochimica Et Biophysica Acta·E FerberW Imm
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
May 1, 1967·Archives of Biochemistry and Biophysics·M R Fenton, K E Richardson
Jul 1, 1947·American Journal of Clinical Pathology·M A ANDERSCH, A J SZCZYPINSKI

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Citations

Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·H J RadzunM R Parwaresch
Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·A C Feller, M R Parwaresch
Aug 1, 1984·European Journal of Pediatrics·G Gaedicke, H G Drexler
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·A C FellerM R Parwaresch
Aug 1, 1986·The Histochemical Journal·H KreipeM R Parwaresch
May 1, 1989·The Histochemical Journal·L H RademakersP I Compier-Spies
Jun 25, 1998·In Vitro Cellular & Developmental Biology. Animal·G GuidaR Cicero
Aug 4, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J RadzunC Sundström
Apr 1, 1982·Immunobiology·M R Parwaresch, H J Radzun
Sep 12, 2014·Thrombosis and Haemostasis·Daniel DuerschmiedIngo Ahrens
Jan 1, 1985·Leukemia Research·H G DrexlerJ Minowada
Dec 1, 1988·Experimental Parasitology·C L Barbieri
Jan 1, 1991·Leukemia & Lymphoma·S M GignacH G Drexler
Sep 15, 1981·Cellular Immunology·H J RadzunJ W Wittke
Mar 1, 1986·Scandinavian Journal of Haematology·H G DrexlerJ Minowada
May 1, 1982·The Journal of Urology·P H WardL Chiang

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