PMID: 6991335Jun 1, 1980Paper

Lysosomal degradation of receptor-bound 125I-labeled insulin by rat adipocytes: its characterization and dissociation from the short-term biologic effects of insulin

Diabetes
G T Hammons, L Jarett

Abstract

In this study we used chloroquine to characterize the interalization and lysosomal degradation of receptor-bound 125I-insulin by rat adipocytes and to determine the role of lysosomal processing of insulin in the short-term biologic effects of the hormone. Chloroquine inhibited the degradation of 125I-insulin bound to adipocytes by both association and disslociation experiments. In the former experiments, chloroquine caused a time- and concentration-dependent increase in specifically bound insulin owing to an increase in intact insulin and a decrease in degradation products, as determined by trichloroacetic acid precipitability and gel chromatographic analysis of material extracted from the cells. In the dissociation experiments, 50 microM chloroquine decreased the rate of degradation by two third, as reflected in the release of degradation to or degraded by isolated plasma membranes, on the degradation of 125I-insulin by proteases in the incubation medium, or on the endocytotic uptake of receptor-bound insulin. Quantitative electron miroscopy, using monomeric ferritin-insulin, showed 50 microM chloroquine doubled the number of lysosomal structures containing ferritin. These findings are consistent with an inhibition by chloroqu...Continue Reading

Citations

Nov 1, 1983·Diabetologia·G W NealW C Duckworth
Dec 1, 1991·Diabetologia·M R Benedict, R A Richman
Apr 1, 1989·Acta diabetologica latina·J C CrestoJ C Basabe
Dec 15, 1981·Biochemical and Biophysical Research Communications·Y IwamotoI D Goldfine
May 30, 1985·Biochimica Et Biophysica Acta·P LönnrothU Smith
Nov 1, 1982·Molecular and Cellular Endocrinology·G CherquiJ Picard
Aug 1, 1985·Molecular and Cellular Endocrinology·R Horuk, J M Olefsky
Nov 24, 1982·Biochimica Et Biophysica Acta·G W Neal, A E Kitabchi
Jan 1, 1988·The International Journal of Biochemistry·S TakahashiH Naito
Jul 9, 1981·The New England Journal of Medicine·A C King, P Cuatrecasas
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D BaldwinJ M Olefsky
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Green, J M Olefsky
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R M Smith, L Jarett
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M P StoppelliM R Rosner
Oct 1, 1981·The Journal of Cell Biology·J L CarpentierL Orci
Nov 1, 1981·Archives of Biochemistry and Biophysics·P A Dennis, N N Aronson
Aug 1, 1981·Metabolism: Clinical and Experimental·B J Goldstein, J N Livingston
Jan 1, 1985·Diabetes/metabolism Reviews·R Horuk, J M Olefsky
May 1, 1983·Journal of Cellular Physiology·R M Smith, L Jarett
Nov 1, 1985·Journal of Cellular Physiology·E WakshullW Wharton
May 1, 1986·Diabetes Research and Clinical Practice·J MossnerJ A Williams
Oct 23, 1997·Biochimica Et Biophysica Acta·W C DuckworthD E Peavy
May 15, 1981·Biochemical and Biophysical Research Communications·D L SeversonA Kryski
Sep 30, 1981·Biochemical and Biophysical Research Communications·S Marshall, J M Olefsky
Jul 1, 1982·Metabolism: Clinical and Experimental·J M OlefskyA Green
Jan 1, 1989·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·R GökeR Arnold
Apr 1, 1988·Journal of Endocrinological Investigation·L BenziR Navalesi
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·Z Zhang, J Radziuk
Feb 1, 1985·The American Journal of Physiology·W G BlackardJ Stillman
Feb 1, 1986·The American Journal of Physiology·W G BlackardL Jarett

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