Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages. Enrichment of cathepsin H in early endosomes.

The Journal of Biological Chemistry
V ClausGareth Griffiths

Abstract

In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well as phagosomes of different ages enclosing 1-micron latex beads to investigate the steady state distribution and trafficking of lysosomal enzyme activity between these organelles. At steady state these cells appear to possess four different cellular structures, in addition to phagolysosomes, where acid hydrolases were concentrated. The first site of hydrolase concentration was the early endosomes, which contained the bulk of the cellular cathepsin H. This enzyme was acquired by phagosomes significantly faster than the other hydrolases tested. The second distinct site of lysosomal enzyme concentration was the late endosomes which contain the bulk of cathepsin S. The third and fourth large pools of hydrolases were found in two functionally distinct types of dense lysosomes, only one of which was found to be secreted in the presence of chloroquine or bafilomycin. Among this secreted pool was soluble furin, generally considered only as a membrane-bound trans-Golgi network resident protein. Thus, the organelles usually referred to as "lysosomes" in fact encompa...Continue Reading

References

May 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y UchiyamaE Kominami
Feb 1, 1990·Archives of Biochemistry and Biophysics·G D Johnson, L B Hersh
Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Jan 1, 1989·Methods in Cell Biology·R B Wilson, R F Murphy
Jan 1, 1988·International Review of Cytology·B Storrie
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y P Deng, B Storrie
Jul 1, 1985·Journal of Biochemistry·E KominamiN Katunuma
Apr 1, 1970·Experimental and Molecular Pathology·R Abraham, R Hendy
Dec 15, 1983·European Journal of Biochemistry·C de Duve
Jun 1, 1984·Biochemical Society Transactions·W JessupP Leoni
Jan 1, 1981·Methods in Enzymology·A J Barrett, H Kirschke
Feb 1, 1981·Experimental and Molecular Pathology·W T StauberB A Schottelius
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·V Y ReddyS J Weiss
Apr 15, 1995·The Biochemical Journal·T BergO Bakke
Jun 30, 1995·The Journal of Biological Chemistry·F AuthierJ J Bergeron
Jan 1, 1994·Methods in Enzymology·H Kirschke, B Wiederanders
Jul 1, 1994·The Journal of Cell Biology·A HaasW Wickner
Mar 1, 1996·The Journal of Experimental Medicine·G RaposoH J Geuze
Apr 7, 1997·The Journal of Cell Biology·A RodríguezN W Andrews
May 1, 1995·Trends in Cell Biology·M Desjardins
Mar 1, 1995·Trends in Cell Biology·W BerónP D Stahl

❮ Previous
Next ❯

Citations

Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T E TjelleT Berg
Oct 24, 2002·Bioorganic Chemistry·Brian E CathersRobert M Rydzewski
Mar 29, 2000·Journal of Biochemical and Biophysical Methods·C S ChenR P Haugland
Mar 10, 2000·Biochimica Et Biophysica Acta·B TurkV Turk
Feb 19, 2000·Current Opinion in Immunology·R J Riese, H A Chapman
Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Jan 19, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rosemary L KanastyDaniel G Anderson
Mar 14, 2007·Nature Cell Biology·Carolina JancicSebastian Amigorena
Apr 17, 2009·Nature Reviews. Microbiology·Ronald S FlannaganSergio Grinstein
Apr 20, 2002·The Biochemical Journal·Ché S PillayClive Dennison
Feb 24, 2001·Cellular Microbiology·S Duclos, M Desjardins
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wenqing ShuiCarolyn R Bertozzi
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Young-wook JunA Paul Alivisatos
Dec 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bahram KasmapourMaximiliano Gabriel Gutierrez
Dec 4, 2012·The Journal of Biological Chemistry·Zala JevnikarJanko Kos
Aug 21, 2002·The Journal of Experimental Medicine·Ana-Maria Lennon-DuménilCécile Lagaudrière-Gesbert
Apr 16, 2002·Molecular Biology of the Cell·Hélène DefacqueGareth Griffiths
Feb 13, 2001·Molecular Biology of the Cell·A JahrausG Griffiths
Sep 13, 2001·Molecular Biology of the Cell·A Al-HaddadS A Kuznetsov
Oct 22, 2002·Molecular Biology of the Cell·Daniel GotthardtThierry Soldati
Jan 19, 2000·The EMBO Journal·H DefacqueG Griffiths
Aug 3, 2001·The EMBO Journal·A M Lennon-DuménilP Wolf Bryant
Mar 9, 1999·Alcoholism, Clinical and Experimental Research·A SlomianyB L Slomiany
Feb 25, 2009·Infection and Immunity·Daniel L ClemensMarcus A Horwitz
Jan 24, 2003·Infection and Immunity·O Ibrahim-GranetJ P Latgé
Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
May 30, 2002·American Journal of Respiratory Cell and Molecular Biology·Frank BraschFrank Bühling
Jun 29, 1999·Biological Chemistry·E KrepelaB Kárová
Nov 20, 2002·Biological Chemistry·Boris TurkVito Turk
Mar 28, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yasuhiro Tsume, Gordon L Amidon
Jan 28, 2014·Nature Nanotechnology·Leo Y T ChouWarren C W Chan
Jan 28, 2014·Microbial Pathogenesis·Philip M IrelandMitali Sarkar-Tyson
Sep 14, 2011·Annual Review of Pathology·Ronald S FlannaganSergio Grinstein
Jun 6, 2009·Cellular and Molecular Life Sciences : CMLS·Hervé Hillaireau, Patrick Couvreur
Jan 10, 2001·The Journal of Cell Biology·J GarinM Desjardins

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