Lysosome fusion to the cell membrane is mediated by the dysferlin C2A domain in coronary arterial endothelial cells.

Journal of Cell Science
Wei-Qing HanPin-Lan Li

Abstract

Dysferlin has recently been reported to participate in cell membrane repair in muscle and other cells through lysosome fusion. Given that lysosome fusion is a crucial mechanism that leads to membrane raft clustering, the present study attempted to determine whether dysferlin is involved in this process and its related signalling, and explores the mechanism underlying dysferlin-mediated lysosome fusion in bovine coronary arterial endothelial cells (CAECs). We found that dysferlin is clustered in membrane raft macrodomains after Fas Ligand (FasL) stimulation as detected by confocal microscopy and membrane fraction flotation. Small-interfering RNA targeted to dysferlin prevented membrane raft clustering. Furthermore, the translocation of acid sphingomyelinase (ASMase) to membrane raft clusters, whereby local ASMase activation and ceramide production--an important step that mediates membrane raft clustering--was attenuated. Functionally, silencing of the dysferlin gene reversed FasL-induced impairment of endothelium-dependent vasodilation in isolated small coronary arteries. By monitoring fluorescence quenching or dequenching, silencing of the dysferlin gene was found to almost completely block lysosome fusion to plasma membrane up...Continue Reading

References

May 30, 1998·Journal of Biochemistry·K UchidaM Umeda
Sep 9, 1998·American Journal of Respiratory Cell and Molecular Biology·S VarsanoJ Radnay
Apr 18, 2002·The Journal of Biological Chemistry·Dawn Belt DavisElizabeth M McNally
Aug 19, 2003·Journal of Hypertension·Lisa BrandinHelena Gustafsson
Aug 21, 2003·Trends in Molecular Medicine·Katherine R Doherty, Elizabeth M McNally
Aug 26, 2003·FEBS Letters·Dongmin ShaoLodewijk V Dekker
Sep 25, 2003·The Journal of Biological Chemistry·Niall J LennonRobert H Brown
Jan 29, 2005·Journal of Clinical Pathology·G CenacchiC Angelini
Apr 13, 2005·Journal of Proteome Research·Aly KarsanTimothy Veenstra
Dec 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erich Gulbins, Pin Lan Li
Feb 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Fan ZhangPin-Lan Li
Feb 21, 2006·Biochemical and Biophysical Research Communications·Yingmei ChaiHerbert Y Gaisano
Aug 4, 2006·American Journal of Physiology. Renal Physiology·Ningjun LiPin-Lan Li
Sep 8, 2006·Journal of Neuropathology and Experimental Neurology·Sonja HochmeisterHans Lassmann
Sep 21, 2006·The Journal of Biological Chemistry·Anna K McNeilPaul L McNeil
Jul 4, 2007·The Journal of Clinical Investigation·Renzhi HanKevin P Campbell
Jul 10, 2007·Nature Cell Biology·Zhijun ZhangShumin Duan
Jul 31, 2007·Antioxidants & Redox Signaling·Pin-Lan LiFan Yi
Dec 29, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Si JinPin-Lan Li
Aug 30, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Su-Jie JiaPin-Lan Li
Sep 6, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Si JinPin-Lan Li
Apr 21, 2010·PloS One·Bilal A AzakirMichael Sinnreich
Dec 4, 2010·Human Molecular Genetics·Alexis R DemonbreunElizabeth M McNally

❮ Previous
Next ❯

Citations

Jun 28, 2012·The Journal of Biological Chemistry·Bilal A AzakirMichael Sinnreich
Nov 21, 2013·International Journal of Molecular Sciences·Isabelle CorreFrançois Paris
Dec 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jaclyn P KerrRobert J Bloch
Jan 15, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Wei-Qing HanPing-Jin Gao
Mar 27, 2013·The FEBS Journal·Andrew MarianoRenzhi Han
Oct 10, 2015·Cell Death and Differentiation·S C SreetamaJ K Jaiswal
Jul 27, 2015·Biochimica Et Biophysica Acta·Eric Boucher, Craig A Mandato
Jul 2, 2016·Journal of Neuroinflammation·Mastura MonifDavid A Williams
May 4, 2017·Journal of Vascular Research·Nadine HaaseTobias Haase
Jun 17, 2019·Journal of Lipid Research·Vanessa R HaynesMatthew J Watt
Feb 29, 2020·Cells·Anna V Bulankina, Sven Thoms
Jun 2, 2017·The Journal of Physiology·Valeriy LukyanenkoRobert J Bloch
Aug 25, 2019·Cells·Olivier PeulenVincent Castronovo
Jul 28, 2018·Cellular and Molecular Life Sciences : CMLS·Adam Horn, Jyoti K Jaiswal
Aug 23, 2020·Cells·Daniel C BittelJyoti K Jaiswal
Apr 5, 2021·Cell Calcium·Srinivasan SundararajMarco G Casarotto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.