Lysosomes co-localize with ryanodine receptor subtype 3 to form a trigger zone for calcium signalling by NAADP in rat pulmonary arterial smooth muscle

Cell Calcium
Nicholas P KinnearA Mark Evans

Abstract

In arterial myocytes the Ca(2+) mobilizing messenger NAADP evokes spatially restricted Ca(2+) bursts from a lysosome-related store that are subsequently amplified into global Ca(2+) waves by Ca(2+)-induced Ca(2+)-release from the sarcoplasmic reticulum (SR) via ryanodine receptors (RyRs). Lysosomes facilitate this process by forming clusters that co-localize with a subpopulation of RyRs on the SR. We determine here whether RyR subtypes 1, 2 or 3 selectively co-localize with lysosomal clusters in pulmonary arterial myocytes using affinity purified specific antibodies. The density of: (1) alphalgP120 labelling, a lysosome-specific protein, in the perinuclear region of the cell (within 1.5mum of the nucleus) was approximately 4-fold greater than in the sub-plasmalemmal (within 1.5mum of the plasma membrane) and approximately 2-fold greater than in the extra-perinuclear (remainder) regions; (2) RyR3 labelling within the perinuclear region was approximately 4- and approximately 14-fold greater than that in the extra-perinuclear and sub-plasmalemmal regions, and approximately 2-fold greater than that for either RyR1 or RyR2; (3) despite there being no difference in the overall densities of fluorescent labelling of lysosomes and RyR s...Continue Reading

References

May 1, 1991·Pflügers Archiv : European journal of physiology·A Herrmann-FrankG Meissner
Jun 1, 1985·The Journal of Cell Biology·V LewisI Mellman
Oct 24, 1995·Biochemical and Biophysical Research Communications·C B NeylonA Bobik
Jun 20, 1998·The Journal of Biological Chemistry·L H JeyakumarS Fleischer
Mar 10, 2001·Biochemical and Biophysical Research Communications·L H JeyakumarS Fleischer
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Mar 13, 2003·Journal of Muscle Research and Cell Motility·Loice H JeyakumarSidney Fleischer
Dec 9, 2003·The Journal of Biological Chemistry·Michiko YamasakiAntony Galione
May 3, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiao-Ru YangJames S K Sham

❮ Previous
Next ❯

Citations

May 29, 2009·Pflügers Archiv : European journal of physiology·Antony GalioneMichael X Zhu
Jun 16, 2010·The Journal of Biological Chemistry·Nezahat Tugba Durlu-KandilciAntony Galione
Aug 20, 2010·The Journal of Biological Chemistry·Samantha J PittRebecca Sitsapesan
Dec 17, 2008·Current Opinion in Nephrology and Hypertension·William J Arendshorst, Tiffany L Thai
Nov 5, 2010·Cold Spring Harbor Perspectives in Biology·Antony Galione
Mar 23, 2010·The Journal of Pharmacology and Experimental Therapeutics·Parvinder K AleyGrant C Churchill
May 7, 2010·American Journal of Physiology. Cell Physiology·Thirumalini VaithianathanAlejandro M Dopico
May 15, 2009·American Journal of Physiology. Renal Physiology·Tiffany L ThaiWilliam J Arendshorst
Jun 3, 2014·Cell Calcium·Gustavo J S PereiraSoraya S Smaili
Apr 3, 2014·Cardiovascular Research·Guillaume GilbertJean-François Quignard
Jun 18, 2014·Cardiovascular Research·Jesus Prieto-Lloret, Philip I Aaronson
Aug 12, 2014·Pharmacology & Therapeutics·Andrea OlschewskiHorst Olschewski
Jun 19, 2013·The Journal of Physiology·Michelle J ConnollyPhilip I Aaronson
Jan 22, 2011·Cell Calcium·Anthony J Morgan
Oct 23, 2009·Annals of the New York Academy of Sciences·A Mark EvansOluseye Ogunbayo
Dec 24, 2010·Cardiovascular Therapeutics·Elisa VenturiRebecca Sitsapesan
Apr 8, 2014·Acta Physiologica·J Parrington, R Tunn
Oct 14, 2011·The Biochemical Journal·Anthony J MorganAntony Galione
Sep 20, 2011·Seminars in Cell & Developmental Biology·Vera KoniecznyColin W Taylor
Sep 6, 2011·Molecular and Cellular Endocrinology·Colin W Taylor, Philippa Dale
Jun 12, 2010·Communicative & Integrative Biology·Michael X ZhuAntony Galione
Jun 19, 2013·Biochimica Et Biophysica Acta·Andy K M Lam, Antony Galione
Aug 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Suengwon LeeJames S K Sham
May 16, 2009·Journal of Pharmacological Sciences·Akitoshi OhnoYuji Imaizumi
Oct 6, 2017·Autophagy·Artem KondratskyiNatalia Prevarskaya
Jan 20, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jesus Prieto-LloretPhilip I Aaronson
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
Mar 29, 2014·Pharmacological Reviews·Marie BillaudBrant E Isakson
Dec 19, 2009·American Journal of Physiology. Cell Physiology·Michael X ZhuA Mark Evans
Apr 11, 2018·Science Signaling·Oluseye A OgunbayoA Mark Evans
Jul 6, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Michiko JoJerome W Breslin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.