PMID: 4327573May 1, 1970

Lysosomes in skeletal muscle tissue. Zonal centrifugation evidence for multiple cellular sources

The Journal of Cell Biology
P G Canonico, J W Bird


Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose-0.02 M KCl gradients. Zonal fractions were analyzed for protein, RNA, cytochrome oxidase, and up to six acid hydrolases. The results indicate the presence of two groups of lysosome-like particles. One group contributes approximately 95% of the cathepsin D and acid phosphatase activity and 75% of the acid ribonuclease, beta-glucuronidase, and arylsulfatase activity in muscle. It is characterized by a modal equilibrium density of 1.18 that is decreased by starvation, but is not shifted by dextran-500 or Triton WR-1339. The second group has a higher proportion of acid ribonuclease, beta-glucuronidase, and arylsulftase; the equilibrium density can be shifted by dextran-500 and Triton WR-1339. It is suggested that this group of lysosomes is derived from macrophages and other connective tissue cells, whereas the former group represents lysosome-like particles from muscle cells.


Jul 1, 1978·Muscle & Nerve·N C Kar, C M Pearson
Oct 20, 1978·European Journal of Applied Physiology and Occupational Physiology·V VihkoA O Ajiduah
Jan 1, 1981·European Journal of Applied Physiology and Occupational Physiology·A Salminen, V Vihko
Jan 1, 1986·Histochemistry·T OkadaM J Karnovsky
Nov 12, 1976·Histochemistry·Z Lojda, E Gutmann
Dec 28, 1978·Pflügers Archiv : European journal of physiology·V VihkoJ Rantamäki
Jul 1, 1979·The Histochemical Journal·J J TroutB A Schottelius
Jan 1, 1983·The Histochemical Journal·W T Stauber, S H Ong
Sep 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·N C Kar, C M Pearson
Jul 1, 1977·Experimental Neurology·M J Cullen, M G Pluskal
Jan 1, 1983·The International Journal of Biochemistry·R A Lawrie
Aug 1, 1977·Journal of the Neurological Sciences·J S Neerunjun, V Dubowitz
Sep 1, 1990·Journal of the Neurological Sciences·L Maltz, U Oron
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·A SalminenV Vihko
Jan 1, 1987·Comparative Biochemistry and Physiology. A, Comparative Physiology·A L GoldembergM Crupkin
Mar 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A Milanesi, J W Bird
Mar 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A Milanesi, J W Bird
Dec 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L H Yamaoka, D M Skinner
Mar 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W T StauberJ W Bird
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G Fanò, A Orlacchio
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Salminen, V Vihko
Oct 1, 2000·Meat Science·M C HughesE E O'Neill
Jan 1, 1978·CRC Critical Reviews in Food Science and Nutrition·A Asghar, N T Yeates
Feb 1, 1972·The Journal of Cell Biology·W A BuschA Suzuki
Jun 1, 1976·European Journal of Biochemistry·M J Warburton, C H Wynn
Oct 1, 1975·The Journal of Clinical Investigation·M G GottwikW B Weglicki
Feb 1, 1977·Journal of Neurochemistry·P MaskreyR J Pennington
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·J L Griffin
Jan 1, 1981·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·J J TroutB A Schottelius
Mar 1, 1977·Journal of Ultrastructure Research·K N Christie, P J Stoward
Apr 1, 1972·Journal of Ultrastructure Research·S Schiaffino, V Hanzlíková
May 1, 1977·Experimental Neurology·W T StauberB A Schottelius


Nov 1, 1969·The Journal of Cell Biology·P G Canonico, J W Bird
Nov 1, 1967·Analytical Biochemistry·N G Anderson, E Rutenberg
Jan 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·J W BirdJ H Leathem
Nov 12, 1968·Biochimica Et Biophysica Acta·N Stagni, B De Bernard
Mar 18, 1969·Biochimica Et Biophysica Acta·E Heyde, J F Morrison
May 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D M Maeir, H Zaiman
Feb 1, 1967·The Journal of Cell Biology·W E Bowers, C de Duve
Feb 1, 1962·Archives of Biochemistry and Biophysics·A L TAPPELS SHIBKO
May 7, 1963·Biochimica Et Biophysica Acta·S SHIBKO, A L TAPPEL
Dec 1, 1963·The Journal of Experimental Medicine·Z A COHN, E WIENER

Related Concepts

Corticosterone Assay
Acid Phosphatase
Cytochrome C Oxidase
Cytochrome c Oxidase Subunit VIa
Lysosome Assembly Pathway
Study, Methodological
Muscle Cells
Gene Products, Protein
Tissue Extracts

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